Human plasma gels: Their preparation and rheological characterization for cell culture applications in tissue engineering
| Autor: | Marta Pevida, Mario Díaz, Sara Llames, Alvaro Meana, Manuel Rendueles, Eva García-Pérez, Ismael Marcet, Lucía Barreda |
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| Rok vydání: | 2019 |
| Předmět: |
Tissue transglutaminase
Scanning electron microscope Cell Culture Techniques Biomedical Engineering Biocompatible Materials 02 engineering and technology Biomaterials Extracellular matrix Plasma 03 medical and health sciences 0302 clinical medicine Tissue engineering Cell Adhesion medicine Humans Fibroblast Cell Proliferation Transglutaminases Tissue Engineering biology Chemistry 030206 dentistry Fibroblasts 021001 nanoscience & nanotechnology Blood proteins medicine.anatomical_structure Mechanics of Materials Cell culture Covalent bond biology.protein Biophysics Rheology 0210 nano-technology Gels |
| Zdroj: | Journal of the Mechanical Behavior of Biomedical Materials. 89:107-113 |
| ISSN: | 1751-6161 |
| DOI: | 10.1016/j.jmbbm.2018.09.015 |
| Popis: | Tissue engineering is one of the fields of clinical medicine that has forged ahead in recent years, especially because of its role as a potential alternative to organ transplantation. The main aim of this study has been the development of biocompatible materials to form extracellular matrix (ECM) structures in order to provide the necessary conditions for the settlement, proliferation and differentiation of dermal cells such as fibroblasts. To this end, human plasma gels were synthesized with the addition of increasing concentrations of transglutaminase (TGase), which catalyses the formation of covalent bonds between Lys and Glu residues. These materials were structurally characterized using rheology and texturometry and were found to have good structural resistance and elasticity for fibroblast culture. A remarkable improvement in the mechanical properties of the human plasma gels was detected when the two highest TGase concentrations were tested, which may be interpreted as an increase in the number of covalent and non-covalent bonds formed between the plasma protein chains. Furthermore, a human fibroblast primary culture was seeded on human plasma scaffolds and satisfactorily proliferated at 37 °C. This was verified in the images obtained by optical microscopy (OM) and by scanning electron microscopy (SEM), which confirmed that the structure of this type of material is suitable for the growth and proliferation of dermal fibroblasts. |
| Databáze: | OpenAIRE |
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