The prototypical Epstein-Barr virus-transformed lymphoblastoid cell line IB4 is an unusual variant containing integrated but no episomal viral DNA
| Autor: | J B Lawrence, L D Klaman, David A. Thorley-Lawson, E A Hurley, S Agger |
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| Rok vydání: | 1991 |
| Předmět: |
Herpesvirus 4
Human Immunology Restriction Mapping medicine.disease_cause Microbiology Virus Herpesviridae Cell Line Nucleic acid thermodynamics Restriction map Plasmid Virology hemic and lymphatic diseases medicine Gammaherpesvirinae Humans Southern blot B-Lymphocytes biology Chromosome Mapping Nucleic Acid Hybridization biology.organism_classification Cell Transformation Viral Epstein–Barr virus Molecular biology Blotting Southern Insect Science DNA Viral Chromosomes Human Pair 4 Plasmids Research Article |
| Zdroj: | Journal of virology. 65(7) |
| ISSN: | 0022-538X |
| Popis: | IB4 is a prototype, latently Epstein-Barr virus (EBV)-infected, lymphoblastoid cell line. We show here that IB4 contains only integrated EBV genomes. Episomal EBV DNA is not detected by Gardella gel analysis or in situ hybridization. Restriction enzyme mapping indicates that the EBV genomes first circularized and then integrated into and deleted part of the BamHI C fragment. IB4 is therefore the only lymphoblastoid cell line described to date that lacks episomal EBV and has integrated EBV genomes with joined ends. Thus, the detection of joined EBV termini on Southern blots is not as reliable as the Gardella gel system for detecting episomal EBV DNA, and IB4 is not an ideal prototype cell line for the study of latent infection by EBV. |
| Databáze: | OpenAIRE |
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