Immunostimulatory response induced by supplementation of Ficus benghalensis root powder, in the artificial feed the Indian freshwater murrel, Channa punctatus
| Autor: | Om Prakash, Neeta Sehgal, Vipin Kumar Verma, Kumari Vandana Rani |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Phagocytosis
Ficus benghalensis Enzyme-Linked Immunosorbent Assay Aquatic Science medicine.disease_cause Plant Roots Nitric oxide chemistry.chemical_compound Adjuvants Immunologic Botany Escherichia coli medicine Animals Environmental Chemistry Food science biology Super oxide dismutase General Medicine Ficus biology.organism_classification Animal Feed Immunity Innate Aeromonas hydrophila Anti-Bacterial Agents Perciformes chemistry biology.protein Plant Preparations Antibody Antibacterial activity Blood Chemical Analysis |
| Zdroj: | Fish & Shellfish Immunology. 33:590-596 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2012.06.031 |
| Popis: | Methanol extract from the dried aerial root of Ficus benghalensis, was used to evaluate antibacterial activity on the bacterial strains of Aeromonas hydrophila and Escherichia coli, by disc diffusion method. In order to study, if there is any immunostimulatory response of F. benghalensis, immunized fish were fed with supplementary artificial feed containing 5% F. benghalensis dried root powder. There was no marked difference in the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in control and treated fish, suggesting that the supplementary feed had no adverse effect on liver or kidney. Serum lysozyme, tissue super oxide dismutase (SOD), percentage phagocytosis, phagocytotic index, nitric oxide (NO), total serum protein and immunoglobulin increased significantly in the treated fish compared to control fish. Serum immunoglobulin levels were estimated by development of a sandwich ELISA, and levels were found to increase with successive immunizations of BSA. |
| Databáze: | OpenAIRE |
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