Cellular differentiation and I-FABP protein expression modulate fatty acid uptake and diffusion
| Autor: | John B. Roths, Andrey Frolov, Barbara P. Atshaves, Friedhelm Schroeder, Jorge A. Piedrahita, Ann B. Kier, William B. Foxworth, Betty Oetama |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Physiology
Cellular differentiation Restriction Mapping Nerve Tissue Proteins Biology Fatty Acid-Binding Proteins Myelin P2 Protein Transfection Polymerase Chain Reaction Fatty acid-binding protein Diffusion Mice Stearates Animals Cells Cultured chemistry.chemical_classification Stem Cells Fatty Acids Fatty acid Fluorescence recovery after photobleaching Cell Differentiation Cell Biology Embryonic stem cell Clone Cells Neoplasm Proteins 4-Chloro-7-nitrobenzofurazan chemistry Biochemistry Indicators and Reagents lipids (amino acids peptides and proteins) Stem cell Carrier Proteins Fatty Acid-Binding Protein 7 Intracellular |
| Zdroj: | American Journal of Physiology-Cell Physiology. 274:C633-C644 |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.1998.274.3.c633 |
| Popis: | The effect of cellular differentiation on fatty acid uptake and intracellular diffusion was examined in transfected pluripotent mouse embryonic stem (ES) cells stably expressing intestinal fatty acid binding protein (I-FABP). Control ES cells, whether differentiated or undifferentiated, did not express I-FABP. The initial rate and maximal uptake of the fluorescent fatty acid, 12-( N-methyl)- N-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-octadecanoic acid (NBD-stearic acid), was measured in single cells by kinetic digital fluorescence imaging. I-FABP expression in undifferentiated ES cells increased the initial rate and maximal uptake of NBD-stearic acid 1.7- and 1.6-fold, respectively, as well as increased its effective intracellular diffusion constant ( Deff) 1.8-fold as measured by the fluorescence recovery after photobleaching technique. In contrast, ES cell differentiation decreased I-FABP expression up to 3-fold and decreased the NBD-stearic acid initial rate of uptake, maximal uptake, and Deffby 10-, 4.7-, and 2-fold, respectively. There were no significant differences in these parameters between the differentiated control and differentiated I-FABP-expressing ES cell lines. In summary, differentiation and expression of I-FABP oppositely modulated NBD-stearic acid uptake parameters and intracellular diffusion in ES cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|