Intracellular localization of samarium in the lactating mammary gland cells: Ultrastructural and microanalytical study
| Autor: | Jaafoura Mohamed-Habib, Audinot Jean-Nicolas, El Hili Ali, Tekaya Leila, Ayadi Ahlem, Maghraoui Samira, Migeon Henri-Noël |
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| Rok vydání: | 2011 |
| Předmět: |
Histology
Cell Mammary gland Spectrometry Mass Secondary Ion chemistry.chemical_element Spleen Mammary Glands Animal Microscopy Electron Transmission medicine Animals Lactation Rats Wistar Instrumentation Samarium Histocytochemistry Anatomy Subcellular localization Molecular biology Rats Medical Laboratory Technology medicine.anatomical_structure chemistry Ultrastructure Female Bone marrow Lysosomes Intracellular |
| Zdroj: | Microscopy Research and Technique. 75:448-451 |
| ISSN: | 1059-910X |
| DOI: | 10.1002/jemt.21076 |
| Popis: | The frequent use of some rare earths in the medical and industrial domains make us worry about their intracellular behavior into the body. Reason for which we have investigated the subcellular localization of one of these elements, the samarium, in the mammary gland of lac- tating female wistar rats using two very sensitive methods of observation and microanalysis, the transmission electron microscopy and the secondary ion mass spectrometry. The ultrastructural study showed the presence of electron dense deposits in the lactating mammary glandular epithe- lial cell lysosomes of the samarium-treated rats, but no loaded lysosomes were observed in those of control rats. The microanalytical study allowed both the identification of the chemical species pres- ent in those deposits as samarium isotopes ( 152 Sm 1 ) and the cartography of its distribution. Our results confirm the previous ones showing that lysosomes of the glandular epithelial cells are the site of the intracellular concentration of foreign elements such as gallium. The intralysosomal deposits observed in the mammary glandular cells of the samarium-treated rats are similar in their form and density to those observed with the same element in other varieties of cells, such as liver, bone marrow, and spleen cells. Our ultrastructural and microanalytical results and those obtained in previous studies allow deducing that the intralysosomal deposits are very probably composed of an insoluble samarium phosphate salt. Microsc. Res. Tech. 75:448-451, 2012. V C 2011 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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