Influence of serum albumin on intracellular delivery of drug-loaded hyaluronan polymeric micelles
Autor: | Jaromír Kulhánek, Lukáš Kubala, Gloria Huerta-Angeles, Kristina Nešporová, Daniela Šmejkalová, Vladimír Velebný, Jana Sogorkova |
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Rok vydání: | 2016 |
Předmět: |
Intracellular Fluid
Cell Survival Polymers Serum albumin Pharmaceutical Science 02 engineering and technology Plasma protein binding 010402 general chemistry 01 natural sciences Micelle chemistry.chemical_compound Drug Delivery Systems Humans Hyaluronic Acid Micelles Serum Albumin Drug Carriers Chromatography biology Nile red Albumin 021001 nanoscience & nanotechnology HCT116 Cells 0104 chemical sciences 3. Good health chemistry Drug delivery biology.protein Nanocarriers 0210 nano-technology Drug carrier HT29 Cells Protein Binding |
Zdroj: | International journal of pharmaceutics. 511(1) |
ISSN: | 1873-3476 |
Popis: | Polymeric micelles are attractive drug delivery systems for intravenously administered nonpolar drugs. Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up. Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin. The composition of micelles affected mainly the loading capacity. Both encapsulated compounds behaved differently in the in vitro cell uptake, which was also influenced by serum concentration, where serum albumin was found to be the primary destabilizing component. This destabilization was found to be influenced by polymeric micelle concentration. Thus, the chemical structure of micelle, the properties of non-covalently loaded substance and serum albumin/polymeric micelle ratio modulate the in vitro intracellular uptake of drugs loaded in nanocarriers. |
Databáze: | OpenAIRE |
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