Novel picornavirus (family Picornaviridae) from freshwater fishes (Perca fluviatilis, Sander lucioperca, and Ameiurus melas) in Hungary
Autor: | Eric Delwart, Eda Altan, Róbert Mátics, Gábor Reuter, Péter Pankovics, Renáta Hargitai, Ákos Boros |
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Rok vydání: | 2021 |
Předmět: |
Viral metagenomics
Picornavirus Zoology Fresh Water Genome Viral Picornaviridae Feces Viral Proteins 03 medical and health sciences Annotated Sequence Record Genus Virology Animals Amino Acid Sequence 3' Untranslated Regions Phylogeny 030304 developmental biology Genomic organization Hungary 0303 health sciences Picornaviridae Infections Lepidotrigla biology 030306 microbiology General Medicine Ameiurus biology.organism_classification Ictaluridae Perches GenBank Freshwater fish RNA Viral 5' Untranslated Regions Sequence Analysis |
Zdroj: | Archives of Virology |
ISSN: | 1432-8798 0304-8608 |
Popis: | In this study, a novel picornavirus (perchPV/M9/2015/HUN, GenBank accession no. MW590713) was detected in eight (12.9%) out of 62 faecal samples collected from three (Perca fluviatilis, Sander lucioperca, and Ameiurus melas) out of 13 freshwater fish species tested and genetically characterized using viral metagenomics and RT-PCR methods. The complete genome of perchPV/M9/2015/HUN is 7,741 nt long, excluding the poly(A) tail, and has the genome organization 5’UTRIRES-?/P1(VP0-VP3-VP1)/P2(2A1NPG↓P-2A2H-box/NC-2B-2C)/P3(3A-3BVPg-3CPro-3DPol)/3’UTR-poly(A). The P1, 2C, and 3CD proteins had 41.4%, 38.1%, and 47.3% amino acid sequence identity to the corresponding proteins of Wenling lepidotrigla picornavirus (MG600079), eel picornavirus (NC_022332), and Wenling pleuronectiformes picornavirus (MG600098), respectively, as the closest relatives in the genus Potamipivirus. PerchPV/M9/2015/HUN represents a potential novel fish-origin species in an unassigned genus in the family Picornaviridae. |
Databáze: | OpenAIRE |
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