In vivo single-particle imaging of nuclear mRNA export in budding yeast demonstrates an essential role for Mex67p
| Autor: | Thomas Dange, Aviva Joseph, Daniel Zenklusen, Karsten Weis, Carlas Smith, David Grunwald, Ammeret Rossouw, Azra Lari, Anette Ouwehand, Ben Montpetit, Carina Patrizia Derrer, Mark Hopman, Maximiliaan Huisman |
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| Rok vydání: | 2015 |
| Předmět: |
Nucleocytoplasmic Transport Proteins
Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae RNA transport RNA-binding protein Biology RNA Transport Report medicine RNA Messenger Nuclear pore Nuclear protein Research Articles Cell Nucleus fungi Nuclear Proteins RNA-Binding Proteins RNA Fungal Cell Biology biology.organism_classification Cell biology Messenger RNP Cell nucleus medicine.anatomical_structure |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Single-particle imaging in budding yeast demonstrates that mRNP export is fast (∼200 ms) and that mRNPs are retained at NPCs and undergo retrograde transport in a mex67-5 mutant, proving an essential role for Mex67p in directional mRNP transport. Many messenger RNA export proteins have been identified; yet the spatial and temporal activities of these proteins and how they determine directionality of messenger ribonucleoprotein (mRNP) complex export from the nucleus remain largely undefined. Here, the bacteriophage PP7 RNA-labeling system was used in Saccharomyces cerevisiae to follow single-particle mRNP export events with high spatial precision and temporal resolution. These data reveal that mRNP export, consisting of nuclear docking, transport, and cytoplasmic release from a nuclear pore complex (NPC), is fast (∼200 ms) and that upon arrival in the cytoplasm, mRNPs are frequently confined near the nuclear envelope. Mex67p functions as the principal mRNP export receptor in budding yeast. In a mex67-5 mutant, delayed cytoplasmic release from NPCs and retrograde transport of mRNPs was observed. This proves an essential role for Mex67p in cytoplasmic mRNP release and directionality of transport. |
| Databáze: | OpenAIRE |
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