A New Barley Stripe Mosaic Virus Allows Large Protein Overexpression for Rapid Function Analysis
| Autor: | Mario Houde, Arnaud Cheuk |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Barley stripe mosaic virus Physiology Genetic Vectors Green Fluorescent Proteins Plant Science Genetically modified crops Computational biology Biology 01 natural sciences Plant Viruses 03 medical and health sciences Cotransformation Genetics Gene silencing Gene Triticum Plant Proteins 2. Zero hunger food and beverages Correction biology.organism_classification Plants Genetically Modified Genetically modified organism 030104 developmental biology Genetic Techniques Functional genomics Function (biology) 010606 plant biology & botany |
| Zdroj: | Plant physiology. 176(3) |
| ISSN: | 1532-2548 |
| Popis: | Understanding the genetic and molecular bases of gene function is of increasing importance to harness their potential to produce plants with novel traits. One important objective is the improvement of plant productivity to meet future demands in food crop production. Gene function is mostly characterized through overexpression or silencing in transgenic plants. This approach is a lengthy procedure, especially in cereals. Plant viral expression systems can be used for rapid expression of proteins. However, current systems have a small cargo capacity and have mostly been used for gene silencing. Here, a four-component barley stripe mosaic virus-based system with high cargo capacity was constructed for the rapid and stable expression of recombinant proteins in different plant species, allowing function analyses at different stages of development. Fluorescent marker proteins are expressed at high levels within 1 week, and a proof of efficient function analysis is shown using the aluminum malate transporter1 gene. In addition to the ability of gene cotransformation, this work demonstrates that the four-component barley stripe mosaic virus-based system allows the overexpression of cDNAs of up to 2,100 nucleotides (encoding a protein of ∼78 kD), thereby providing an invaluable tool to accelerate functional genomics and proteomic research in monocot and dicot species. |
| Databáze: | OpenAIRE |
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