Human menstrual blood-derived mesenchymal stem cells regulation of the EGF/Ras p21 pathway as a potential therapeutic target for thin endometrium

Autor:	Kunming Li, Xiaoming Teng, Fengli Chi, Mei Zhao, Tingyu Zhang
Rok vydání:	2021
Předmět:	Angiogenesis medicine.medical_treatment Mesenchymal stem cell General Medicine Endometrial Disorder Stem-cell therapy Biology Endometrium Stem cell marker medicine.anatomical_structure medicine Cancer research Original Article Stem cell Progenitor cell
Zdroj:	Ann Transl Med
ISSN:	2305-5847 2305-5839
DOI:	10.21037/atm-21-4652
Popis:	BACKGROUND: Human infertility is caused by many factors, among which thin endometrium is the main reason for poor embryo implantation. Currently, stem cell therapy could be a potential approach in treating human endometrial disorder like thin endometrium. In this study, we aimed to explore the influence of menstrual stem cells from non-thin endometrium (NTE-MenSCs) and thin endometrium (TE-MenSCs) on the phenotype of endometrial epithelial cells (EECs). METHODS: The MenSCs were isolated from women with and without thin endometria, characterized and co-cultured with the EECs. The expression of cytokeratin 7 (CK7) was verified by immunofluorescence while the detection stem cell markers was determined flow cytometry. Osteogenic and adipogenic differentiation were induced in appropriate media. The quantitative real-time PCR and western blotting were respectively used for detecting the mRNA and protein expression levels, respectively. The CCK-8 assay was used for cell viability analysis whereas ELISA was used for the detection of cytokine levels. RESULTS: The results showed that the co-culture of NTE-MenSCs or TE-MenSCs and EECs promoted the proliferation, migration, and angiogenesis of endothelial progenitor cells differently. Furthermore, the TE-MenSCs promoted the expression of inflammation, vascularized adipose, and extracellular matrix related proteins. The epidermal growth factor (EGF)/Ras p21 pathway was found to mediate the influence of MenSCs on EECs. CONCLUSIONS: These findings are vital in that they may promote stem cell therapy of thin endometrium and enable embryo implantation in humans with thin endometrium.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3970391585ce263b5165b8b700d861e7 https://doi.org/10.21037/atm-21-4652 Zobrazit plný text záznamu