Membrane-Protected Molecularly Imprinted Polymer for the Microextraction of Indole-3-butyric Acid in Mung Bean Sprouts
| Autor: | Aziguli Yigaimu, Turghun Muhammad, Parezhati Pataer, Anwar Mamat, Sailemayi Aihebaier, Aixia Wei, Abliz Yimit, Munira Abuduaini |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | ACS Omega ACS Omega, Vol 4, Iss 16, Pp 16789-16793 (2019) |
| ISSN: | 2470-1343 |
| Popis: | Based on the hollow fiber protected molecularly imprinted polymer, a micro-solid-phase extraction (μ-SPE) method was developed and applied for the analysis of indole-3-butyric acid in mung bean sprouts by high-performance liquid chromatography. The extraction conditions of the μ-SPE method were optimized using L9(34) orthogonal, and optimum conditions were found as follows: pH of sample solution was 2.0, chloroform was the organic solvent for embedding the μ-SPE bars, and acetonitrile was the desorption solvent. In addition, the extraction time was 80 min, desorption time was 5 min, stirring speed was 800 rpm, and concentration of NaCl was 10%. Under the optimum conditions, a standard curve was established for IBA, with a correlation coefficient of 0.9999. After extraction with phosphate buffer solution (pH = 9.0), successful pretreatment of mung bean sprouts was achieved by the μ-SPE method. The limit of detection was 0.075 mg/kg, and the recoveries were found to be in the range of 88.9-106.4%. This method is simple, environmentally friendly, and can be used for the determination of indole auxin contents in green bean sprouts quickly and accurately. |
| Databáze: | OpenAIRE |
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