Micropropagation of Sicilian cultivars with an aim to preserve genetic diversity in hazelnut (Corylus avellana L.)
| Autor: | Emilia Caboni, A. Gentile, A. Sgueglia, G. Urbinati, Maria Germana, Andrea Frattarelli |
|---|---|
| Přispěvatelé: | Sgueglia A., Gentile A., Frattarelli A., Urbinati G., Germanà M., Caboni E. |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Biodiversity indole-3-butyric acid Plant Science Biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Cultivar N6-benzyladenine Ecology Evolution Behavior and Systematics Decontamination time Genetic diversity shoot multiplication business.industry Indole-3-butyric acid language.human_language Settore AGR/03 - Arboricoltura Generale E Coltivazioni Arboree Horticulture 030104 developmental biology Micropropagation chemistry rooting induction Agriculture metatopolin language Agricultural biodiversity business Sicilian 010606 plant biology & botany |
| Zdroj: | Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology. 153:720-724 |
| ISSN: | 1724-5575 1126-3504 |
| DOI: | 10.1080/11263504.2018.1549600 |
| Popis: | The use of a small number of cultivars in agriculture can lead to a loss of agrobiodiversity. Since in vitro techniques are valuable tools for conserving plant biodiversity, an efficient micropropagation protocol for four Italian hazelnut cultivars, ‘Carrello’, ‘Ghirara’, ‘Minnulara’, and ‘Panottara’, was developed. The highest axillary bud survival was obtained after decontamination with 40 min 1% sodium hypochlorite followed by 40 min 0.1% sodium merthiolate in ‘Minnulara’ and ‘Ghirara’, while the 35þ35 min treatment was the best for ‘Carrello’ and ‘Panottara’. Shoot multiplication was higher in ‘Minnulara’ and ‘Ghirara’ when 6.6 lM N6-benzyladenine was used, even if some hyperhydric shoots were observed, while metatopolin was more effective in the other two cultivars. In vitro rooting, performed in ‘Carrello’ and ‘Panottara’, was higher with 17.6 than with 9.8 mM indole-3-butyric. Following in vitro root induction with 17.6 mM indole-3-butyric acid for 7 days, rooting and acclimatisation in greenhouse exceeded 85% for all four cultivars. |
| Databáze: | OpenAIRE |
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