Coordination and fine motor control depend on Drosophila TRPγ
| Autor: | Nina Boiko, Qiuting Ren, James D. Stockand, Bradley Akitake, Craig Montell, Jinfei Ni, Takaaki Sokabe, Benjamin A. Eaton |
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| Rok vydání: | 2014 |
| Předmět: |
Patch-Clamp Techniques
General Physics and Astronomy Biology TRPV General Biochemistry Genetics and Molecular Biology Article Animals Genetically Modified Transient receptor potential channel Gene Knockout Techniques Transient Receptor Potential Channels Animals Drosophila Proteins Humans Patch clamp Drosophila (subgenus) Motor skill TRPC Multidisciplinary Sodium General Chemistry Anatomy biology.organism_classification Proprioception Microscopy Electron Drosophila melanogaster HEK293 Cells Motor Skills Calcium Neuroscience Mechanoreceptors Drosophila Protein |
| Zdroj: | Nature communications. 6 |
| ISSN: | 2041-1723 |
| Popis: | Motor coordination is broadly divided into gross and fine motor control, both of which depend on proprioceptive organs. However, the channels that function specifically in fine motor control are unknown. Here we show that mutations in trpγ disrupt fine motor control while leaving gross motor proficiency intact. The mutants are unable to coordinate precise leg movements during walking, and are ineffective in traversing large gaps due to an inability in making subtle postural adaptations that are requisite for this task. TRPγ is expressed in proprioceptive organs, and is required in both neurons and glia for gap crossing. We expressed TRPγ in vitro, and found that its activity is promoted by membrane stretch. A mutation eliminating the Na(+)/Ca(2+) exchanger suppresses the gap-crossing phenotype of trpγ flies. Our findings indicate that TRPγ contributes to fine motor control through mechanical activation in proprioceptive organs, thereby promoting Ca(2+) influx, which is required for function. |
| Databáze: | OpenAIRE |
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