Transplantation and survival of mouse inner ear progenitor/stem cells in the organ of Corti after cochleostomy of hearing-impaired guinea pigs: preliminary results

Autor: Ricardo Ferreira Bento, Karina Lezirovitz, Daniela B Zanatta, Jeanne Oiticica, Luciana Caixeta Barboza, Bryan E. Strauss, Luciana Amaral Haddad, Regina Célia Mingroni-Netto
Rok vydání: 2016
Předmět:
0301 basic medicine
Pathology
Physiology
Stem cells
PROCEDIMENTOS CIRÚRGICOS OTOLÓGICOS
Biochemistry
0302 clinical medicine
General Pharmacology
Toxicology and Pharmaceutics
Organ of Corti
lcsh:QH301-705.5
Cells
Cultured
Protein Synthesis Inhibitors
Mice
Inbred BALB C
lcsh:R5-920
General Neuroscience
General Medicine
Immunohistochemistry
Cochlea
Basilar membrane
Treatment Outcome
medicine.anatomical_structure
Female
Sensorineural hearing loss
Hair cell
lcsh:Medicine (General)
medicine.medical_specialty
Cell Survival
Hearing Loss
Sensorineural
Guinea Pigs
Immunology
Biophysics
Biology
03 medical and health sciences
Ototoxicity
Evoked Potentials
Auditory
Brain Stem
otorhinolaryngologic diseases
medicine
Animals
Inner ear
Hair Cells
Auditory
Inner
Biomedical Sciences
Reproducibility of Results
Auditory Threshold
Neomycin
Hearing loss
Cell Biology
medicine.disease
Transplantation
030104 developmental biology
lcsh:Biology (General)
sense organs
Cell transplantation
030217 neurology & neurosurgery
Stem Cell Transplantation
Zdroj: Brazilian Journal of Medical and Biological Research, Vol 49, Iss 4 (2016)
Brazilian Journal of Medical and Biological Research v.49 n.4 2016
Brazilian Journal of Medical and Biological Research
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Brazilian Journal of Medical and Biological Research, Volume: 49, Issue: 4, Article number: e5064, Published: 18 MAR 2016
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 0100-879X
DOI: 10.1590/1414-431x20155064
Popis: In mammals, damage to sensory receptor cells (hair cells) of the inner ear results in permanent sensorineural hearing loss. Here, we investigated whether postnatal mouse inner ear progenitor/stem cells (mIESCs) are viable after transplantation into the basal turns of neomycin-injured guinea pig cochleas. We also examined the effects of mIESC transplantation on auditory functions. Eight adult female Cavia porcellus guinea pigs (250-350 g) were deafened by intratympanic neomycin delivery. After 7 days, the animals were randomly divided in two groups. The study group (n=4) received transplantation of LacZ-positive mIESCs in culture medium into the scala tympani. The control group (n=4) received culture medium only. At 2 weeks after transplantation, functional analyses were performed by auditory brainstem response measurement, and the animals were sacrificed. The presence of mIESCs was evaluated by immunohistochemistry of sections of the cochlea from the study group. Non-parametric tests were used for statistical analysis of the data. Intratympanic neomycin delivery damaged hair cells and increased auditory thresholds prior to cell transplantation. There were no significant differences between auditory brainstem thresholds before and after transplantation in individual guinea pigs. Some mIESCs were observed in all scalae of the basal turns of the injured cochleas, and a proportion of these cells expressed the hair cell marker myosin VIIa. Some transplanted mIESCs engrafted in the cochlear basilar membrane. Our study demonstrates that transplanted cells survived and engrafted in the organ of Corti after cochleostomy.
Databáze: OpenAIRE
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