SerpinB10, a Serine Protease Inhibitor, Is Implicated in UV-Induced Cellular Response
| Autor: | Zsuzsanna Újfaludi, Zoltán Gábor Páhi, Lajos Haracska, Imre Boros, Hajnalka Majoros, Tibor Pankotai, Mónika Mórocz, Barbara N. Borsos |
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| Rok vydání: | 2021 |
| Předmět: |
Cell type
replication Microarray DNA Repair QH301-705.5 DNA repair DNA damage Ultraviolet Rays replication stress serine protease inhibitor medicine.disease_cause Catalysis Article Bomapin S Phase Inorganic Chemistry 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Line Tumor medicine Humans SerpinB10 Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Gene Spectroscopy Serpins 030304 developmental biology Serine protease 0303 health sciences UV damage biology Chemistry Organic Chemistry General Medicine 3. Good health Computer Science Applications Cell biology 030220 oncology & carcinogenesis biology.protein Carcinogenesis DNA Damage |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 16 International Journal of Molecular Sciences, Vol 22, Iss 8500, p 8500 (2021) |
| ISSN: | 1422-0067 |
| Popis: | UV-induced DNA damage response and repair are extensively studied processes, as any malfunction in these pathways contributes to the activation of tumorigenesis. Although several proteins involved in these cellular mechanisms have been described, the entire repair cascade has remained unexplored. To identify new players in UV-induced repair, we performed a microarray screen, in which we found SerpinB10 (SPB10, Bomapin) as one of the most dramatically upregulated genes following UV irradiation. Here, we demonstrated that an increased mRNA level of SPB10 is a general cellular response following UV irradiation regardless of the cell type. We showed that although SPB10 is implicated in the UV-induced cellular response, it has no indispensable function in cell survival upon UV irradiation. Nonetheless, we revealed that SPB10 might be involved in delaying the duration of DNA repair in interphase and also in S-phase cells. Additionally, we also highlighted the interaction between SPB10 and H3. Based on our results, it seems that SPB10 protein is implicated in UV-induced stress as a “quality control protein”, presumably by slowing down the repair process. |
| Databáze: | OpenAIRE |
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