Nociception in a Progressive Multiple Sclerosis Model in Mice Is Dependent on Spinal TRPA1 Channel Activation
Autor: | Gabriele Cheiran Pereira, Maria Fernanda Pessano Fialho, Camila Duarte Ritter, Débora Denardin Lückemeyer, Sabrina Qader Kudsi, Sara Marchesan Oliveira, Caren Tatiane de David Antoniazzi, Gabriela Trevisan, Amanda Spring de Almeida, Juliano Ferreira, Vitória Loreto Pereira, Diéssica Padilha Dalenogare |
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Rok vydání: | 2020 |
Předmět: |
Nociception
0301 basic medicine Dipyrone Pregabalin Pharmacology Mice chemistry.chemical_compound 0302 clinical medicine Oximes TRPA1 Cation Channel Analgesics Thioctic Acid biology Experimental autoimmune encephalomyelitis food and beverages Up-Regulation Allodynia Spinal Cord Neurology Hyperalgesia Neuropathic pain Female medicine.symptom Agonist Encephalomyelitis Autoimmune Experimental medicine.drug_class Neuroscience (miscellaneous) Nerve Tissue Proteins Myelin oligodendrocyte glycoprotein 03 medical and health sciences Cellular and Molecular Neuroscience medicine Animals Neuroinflammation business.industry Acetophenones NADPH Oxidases Oligonucleotides Antisense medicine.disease Peptide Fragments Mice Inbred C57BL Oxidative Stress 030104 developmental biology chemistry Purines Apocynin biology.protein Neuralgia Acetanilides Myelin-Oligodendrocyte Glycoprotein business Antipyrine 030217 neurology & neurosurgery |
Zdroj: | Molecular Neurobiology. 57:2420-2435 |
ISSN: | 1559-1182 0893-7648 |
Popis: | Central neuropathic pain is a common untreated symptom in progressive multiple sclerosis (PMS) and is associated with poor quality of life and interference with patients’ daily activities. The neuroinflammation process and mitochondrial dysfunction in the PMS lesions generate reactive species. The transient potential receptor ankyrin 1 (TRPA1) has been identified as one of the major mechanisms that contribute to neuropathic pain signaling and can be activated by reactive compounds. Thus, the goal of our study was to evaluate the role of spinal TRPA1 in the central neuropathic pain observed in a PMS model in mice. We used C57BL/6 female mice (20–30 g), and the PMS model was induced by the experimental autoimmune encephalomyelitis (EAE) using mouse myelin oligodendrocyte glycoprotein (MOG35–55) antigen and CFA (complete Freund’s adjuvant). Mice developed progressive clinical score, with motor impairment observed after 15 days of induction. This model induced mechanical and cold allodynia and heat hyperalgesia which were measured up to 14 days after induction. The hypersensitivity observed was reduced by the administration of selective TRPA1 antagonists (HC-030031 and A-967079, via intrathecal and intragastric), antioxidants (α-lipoic acid and apocynin, via intrathecal and intragastric), and TRPA1 antisense oligonucleotide (via intrathecal). We also observed an increase in TRPA1 mRNA levels, NADPH oxidase activity, and 4-hydroxinonenal (a TRPA1 agonist) levels in spinal cord samples of PMS-EAE induced animals. In conclusion, these results support the hypothesis of the TRPA1 receptor involvement in nociception observed in a PMS-EAE model in mice. |
Databáze: | OpenAIRE |
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