The interplay between 5-HT2C and 5-HT3A receptors in the dorsal periaqueductal gray mediates anxiety-like behavior in mice

Autor: Rimenez R. Souza, Azair Canto-de-Souza, Luana Tenorio Lopes, Daniela Baptista-de-Souza, Ricardo Luiz Nunes-de-Souza, Lucas Canto-de-Souza
Přispěvatelé: Universidade Federal de São Carlos (UFSCar), Universidade Estadual Paulista (UNESP), Neuroscience and Behavioral Institute, The University of Calgary, School of Behavior and Brain Sciences, Texas Biomedical Device Center
Rok vydání: 2022
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Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 0166-4328
DOI: 10.1016/j.bbr.2021.113588
Popis: Made available in DSpace on 2022-04-28T19:44:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-24 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The monoamine neurotransmitter serotonin (5-HT) modulates anxiety by its activity on 5-HT2C receptors (5-HT2CR) expressed in the dorsal periaqueductal gray (dPAG). Here, we investigated the presence of 5-HT3A receptors (5-HT3AR) in the dPAG, and the interplay between 5-HT2CR and 5-HT3AR in the dPAG in mediating anxiety-like behavior in mice. We found that 5-HT3AR is expressed in the dPAG and the blockade of these receptors using intra-dPAG infusion of ondansetron (5-HT3AR antagonist; 3.0 nmol) induced an anxiogenic-like effect. The activation of 5-HT3ABR by the infusion of mCPBG [1-(m-Chlorophenyl)-biguanide; 5-HT3R agonist] did not alter anxiety-like behaviors. In addition, blockade of 5-HT3AR (1.0 nmol) prevented the anxiolytic-like effect induced by the infusion of the 5-HT2CR agonist mCPP (1-(3-chlorophenyl) piperazine; 0.03 nmol). None of the treatment effects on anxiety-like behaviors altered the locomotor activity levels. The present results suggest that the anxiolytic-like effect exerted by serotonin activity on 5-HT2CR in the dPAG is modulated by 5-HT3AR expressed in same region. Psychobiology Group/Department of Psychology/CECH–UFSCar Joint Graduate Program in Physiological Sciences UFSCar/UNESP, Rod. Washington Luís, Km 235 Graduate Program in Psychology UFSCar, Rod. Washington Luís, Km 235 Laboratory of Pharmacology School of Pharmaceutical Sciences Univ. Estadual Paulista UNESP Neuroscience and Behavioral Institute, Av. do Café, 2.450 Department of Physiology and Pharmacology Hotchkiss Brain Institute The University of Calgary The University of Texas at Dallas School of Behavior and Brain Sciences, 800 West Campbell Road The University of Texas at Dallas Texas Biomedical Device Center, 800 West Campbell Road Joint Graduate Program in Physiological Sciences UFSCar/UNESP, Rod. Washington Luís, Km 235 Laboratory of Pharmacology School of Pharmaceutical Sciences Univ. Estadual Paulista UNESP FAPESP: 2016/08665-0 CNPq: 88887.194785/2018-00
Databáze: OpenAIRE
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