Ultrastructural changes of goat corpus luteum during the estrous cycle
| Autor: | Yi-Fan Jiang, Kuan-Hao Tsui, Meng-Chieh Hsu, Chiung-Hsiang Cheng, Chih-Hsien Chiu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Programmed cell death Angiogenesis Estrous Cycle Luteal phase Biology 03 medical and health sciences Endocrinology Food Animals Corpus Luteum Internal medicine Organelle medicine Animals Estrous cycle Goats Endoplasmic reticulum 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine 040201 dairy & animal science Cell biology 030104 developmental biology medicine.anatomical_structure Ultrastructure Female Animal Science and Zoology Corpus luteum |
| Zdroj: | Animal Reproduction Science. 170:38-50 |
| ISSN: | 0378-4320 |
| DOI: | 10.1016/j.anireprosci.2016.04.001 |
| Popis: | The present study was designed to study the ultrastructure of goat corpora lutea (CL, n=10) and structural changes as related to steroidogenic functions during the estrous cycle. The reproduction status of goats was estimated by analyzing serum progesterone concentrations. The CL at various stages was surgically collected. To characterize ultrastructural features associated with steroidogenesis, tissue and cellular structures were studied. Blood supplies were examined based on features of the endothelial cells and capillary structures in the CL. Activated endothelial cells and developing vessels were observed in the early stage, whereas mature endothelial cells, accumulating extracellular matrix fibers, and stabilized vessels were observed in the middle and late stages of assessment. In the late stage of assessment, shrunken goat luteal cells scattered around the capillaries were detected and formed circular regression areas. Features of autophagy and luteal cell apoptosis were noted. In large luteal cells, steroidogenic organelles were present, including microvillar channels, endoplasmic reticulum, and mitochondria. Conformational changes in the endoplasmic reticulum and increased mitochondria with tubular cristae were observed in the early-middle CL transitions. In contrast, mitochondria swelled and the cristae transformed to the lamellar type in the late stage, suggesting that organelle plasticity could contribute to steroidogenesis in goat CL. In conclusion, results suggest angiogenesis occurs in early developing CL and programmed cell death occurred in the late stage of CL assessment in the present study. Structures and quantiles of steroidogenic organelles are correlated with the steroidogenic functions in goats. |
| Databáze: | OpenAIRE |
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