Platelet-Rich Plasma and Adipose-Derived Mesenchymal Stem Cells for Regenerative Medicine-Associated Treatments in Bottlenose Dolphins (Tursiops truncatus)

Autor: Mario Soriano-Navarro, Maravillas Mellado-López, Victoria Moreno-Manzano, Daniel García-Párraga, Alicia Martínez-Romero, José Luis Crespo-Picazo, Richard J. Griffeth
Rok vydání: 2014
Předmět:
Male
Vascular Endothelial Growth Factor A
Pathology
medicine.medical_specialty
lcsh:Medicine
Adipose tissue
Bioengineering
Industrial Processes
Biology
Regenerative Medicine
Research and Analysis Methods
Regenerative medicine
Complementary and Alternative Medicine
Transforming Growth Factor beta
Industrial Engineering
Blood plasma
Medicine and Health Sciences
medicine
Animals
Humans
Cell Separation Techniques
Platelet
lcsh:Science
Cells
Cultured
Cell Proliferation
Whole blood
Wound Healing
Multidisciplinary
Platelet-Rich Plasma
lcsh:R
Mesenchymal stem cell
Biology and Life Sciences
Cell Differentiation
Mesenchymal Stem Cells
Cell Biology
Bottle-Nosed Dolphin
Separation Processes
Platelet-rich plasma
Animal Studies
Engineering and Technology
lcsh:Q
Female
Veterinary Science
Sanitary Engineering
Biological Cultures
Wound healing
human activities
Research Article
Biotechnology
Zdroj: PLoS ONE
Plos One
r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
instname
PLoS ONE, Vol 9, Iss 9, p e108439 (2014)
r-CIPF: Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
Centro de Investigación Principe Felipe (CIPF)
ISSN: 1932-6203
Popis: Dolphins exhibit an extraordinary capacity to heal deep soft tissue injuries. Nevertheless, accelerated wound healing in wild or captive dolphins would minimize infection and other side effects associated with open wounds in marine animals. Here, we propose the use of a biological-based therapy for wound healing in dolphins by the application of platelet-rich plasma (PRP). Blood samples were collected from 9 different dolphins and a specific and simple protocol which concentrates platelets greater than two times that of whole blood was developed. As opposed to a commonly employed human protocol for PRP preparation, a single centrifugation for 3 minutes at 900 rpm resulted in the best condition for the concentration of dolphin platelets. By FACS analysis, dolphin platelets showed reactivity to platelet cell-surface marker CD41. Analysis by electron microscopy revealed that dolphin platelets were larger in size than human platelets. These findings may explain the need to reduce the duration and speed of centrifugation of whole blood from dolphins to obtain a 2-fold increase and maintain proper morphology of the platelets. For the first time, levels of several growth factors from activated dolphin platelets were quantified. Compared to humans, concentrations of PDGF-BB were not different, while TGF beta and VEGF-A were significantly lower in dolphins. Additionally, adipose tissue was obtained from cadaveric dolphins found along the Spanish Mediterranean coast, and adipose-derived mesenchymal stem cells (ASCs) were successfully isolated, amplified, and characterized. When dolphin ASCs were treated with 2.5 or 5% dolphin PRP they exhibited significant increased proliferation and improved phagocytotic activity, indicating that in culture, PRP may improve the regenerative capacity of ASCs. Taken together, we show an effective and well-defined protocol for efficient PRP isolation. This protocol alone or in combination with ASCs, may constitute the basis of a biological treatment for wound-healing and tissue regeneration in dolphins.
Databáze: OpenAIRE
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