Comparison of multiplex PCR with serogrouping and PCR-RFLP of fliC gene for the detection of enteropathogenic Escherichia coli (EPEC)
| Autor: | Mana Oloomi, Anis Jafari, Amir Dashti, Saeid Bouzari, Mohammad Mehdi Aslani |
|---|---|
| Přispěvatelé: | Molecular Biology Department, Institut Pasteur d'Iran, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Réseau International des Instituts Pasteur (RIIP), This project was financially supported by Pasteur Institute of Iran (grant no: 268). |
| Rok vydání: | 2011 |
| Předmět: |
polymerase chain reaction
lcsh:QR1-502 diarrhea MESH: Escherichia coli Proteins Shiga Toxin 1 Shiga Toxin 2 lcsh:Microbiology law.invention Serology Feces law STX2 MESH: Child Child Polymerase chain reaction Medicine(all) Human feces 0303 health sciences MESH: Multiplex Polymerase Chain Reaction Escherichia coli Proteins MESH: Feces O Antigens enteropathogenic Escherichia coli General Medicine 3. Good health MESH: Diarrhea Diarrhea Infectious Diseases Restriction fragment length polymorphism medicine.symptom Polymorphism Restriction Fragment Length Microbiology (medical) DNA Bacterial Biology lcsh:Infectious and parasitic diseases Microbiology 03 medical and health sciences parasitic diseases Multiplex polymerase chain reaction medicine Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology lcsh:RC109-216 Enteropathogenic Escherichia coli Serotyping MESH: Enteropathogenic Escherichia coli MESH: O Antigens 030304 developmental biology MESH: Humans MESH: Polymorphism Restriction Fragment Length 030306 microbiology MESH: Serotyping MESH: Shiga Toxin 2 MESH: Shiga Toxin 1 MESH: DNA Bacterial [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Virology bacteria Multiplex Polymerase Chain Reaction Flagellin |
| Zdroj: | Brazilian Journal of Infectious Diseases, Vol 15, Iss 4, Pp 365-369 Brazilian Journal of Infectious Diseases, Volume: 15, Issue: 4, Pages: 365-369, Published: AUG 2011 Brazilian Journal of Infectious Diseases, Vol 15, Iss 4, Pp 365-369 (2011) Brazilian Journal of Infectious Diseases Brazilian Journal of Infectious Diseases, Elsevier, 2011, 15 (4), pp.365-369. ⟨10.1016/S1413-8670(11)70206-9⟩ Brazilian Journal of Infectious Diseases v.15 n.4 2011 Brazilian Society of Infectious Diseases (BSID) instacron:BSID |
| ISSN: | 1678-4391 1413-8670 |
| DOI: | 10.1590/s1413-86702011000400011 |
| Popis: | Enteropathogenic Escherichia coli (EPEC) comprise one of the six categories of diarrhoeagenic E. coli (DEC). EPEC is subgrouped into typical (tEPEC) and atypical (aEPEC). The identification of DEC cannot be based only on cultural and biochemical criteria, since they are indistinguishable from the non-pathogenic E. coli commonly found in human feces. Several PCR methods, with both single and multiple target genes, have been reported for detecting the different DEC pathotypes. In the present study five hundred E. coli isolates from children with diarrhea were subjected into multiplex PCR. Furthermore the strains were typed serologically with O antisera and their fliC gene was characterized by PCR-RFLP. The results obtained revealed that overall 41 (8.2%) isolates could be detected as EPEC by this multiplex PCR assay. Of these isolates; 27 (66%) were typical (escv+, bfp+) and 14 (34%) atypical EPEC (escv+, bfp-). None of these 41 isolates contained the Stx1 and Stx2 genes. Among 37 (90%) typeable strains, nine different serogroups were present. The most common serogroups were O111, followed by O86, O55 and O119 and 10 different H types were found among these isolates. The multiplex PCR assay was found to be rapid and reliable in comparison to serological test; especially when screening the large number of isolates. Keywords: diarrhea, enteropathogenic Escherichia coli, polymerase chain reaction |
| Databáze: | OpenAIRE |
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