Effect of profilin‐1 on the asymmetric dimethylarginine‐induced vascular lesion‐associated hypertension
| Autor: | Jin-Fang Cheng, Mei-Fang Chen, Yuan-Jian Li, Tianlun Yang, Guo-Hua Ni, Qiying Xie |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
JAK2/STAT3 pathway
medicine.medical_specialty Medicine (General) Vascular smooth muscle hypertension profilin‐1 proliferation Myocytes Smooth Muscle macromolecular substances asymmetric dimethylarginine (ADMA) Arginine Essential hypertension Nitric oxide Profilins chemistry.chemical_compound R5-920 Downregulation and upregulation Internal medicine Animals Humans Medicine STAT3 Cell Proliferation Janus kinase 2 biology business.industry General Medicine medicine.disease Rats Endocrinology chemistry biology.protein STAT protein Endothelium Vascular Asymmetric dimethylarginine business |
| Zdroj: | Kaohsiung Journal of Medical Sciences, Vol 38, Iss 2, Pp 149-156 (2022) |
| ISSN: | 2410-8650 |
| Popis: | Previous studies have demonstrated that the levels of asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide (NO) synthesis, are strongly associated with hypertension, diabetes, and cardiovascular diseases. Profilin‐1, an actin‐binding protein, has been documented to be involved in endothelial injury and in the proliferation of vascular smooth muscle cells resulting from hypertension. However, the role of profilin‐1 in ADMA‐induced vascular injury in hypertension remains largely unknown. Forty healthy subjects and forty‐two matched patients with essential hypertension were enrolled, and the related indexes of vascular injury in plasma were detected. Rat aortic smooth muscle cells (RASMCs) were treated with different concentrations of ADMA for different periods of time and transfected with profilin‐1 small hairpin RNA to interrupt the expression of profilin‐1. To determine the role of the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway, RASMCs were pretreated with AG490 or rapamycin. The expression of profilin‐1 was tested using real‐time polymerase chain reaction (PCR) and western blot analysis. Cell proliferation was measured by flow cytometry and 3‐(4,5‐dimethylthiazol‐2yl)‐2,5‐diphenyltetrazoliumbromide assays. Compared with healthy subjects, the levels of ADMA and profilin‐1 were markedly elevated in hypertensive individuals, while the levels of NO were significantly decreased (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text