The Use of Ostrich Eggs for In Ovo Research: Making Preclinical Imaging Research Affordable and Available
| Autor: | Ronny Stolz, Thomas Winkens, Ralph Huonker, Otto W. Witte, Tobias Niksch, Thomas Opfermann, Steffen Wiegand, Martin Freesmeyer, Christian Kuehnel |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Fluorine Radioisotopes medicine.medical_specialty Embryo Nonmammalian Contrast Media Embryonic Development In ovo 030218 nuclear medicine & medical imaging Iodine Radioisotopes 03 medical and health sciences Preclinical research Imaging Three-Dimensional 0302 clinical medicine Fluorodeoxyglucose F18 Motion artifacts Positron Emission Tomography Computed Tomography Animals Medicine Radiology Nuclear Medicine and imaging Ovum Struthioniformes business.industry Pet imaging Contrast medium 030104 developmental biology embryonic structures Ct scanners Female Radiology Radiopharmaceuticals Tomography X-Ray Computed business Preclinical imaging |
| Zdroj: | Journal of Nuclear Medicine. 59:1901-1906 |
| ISSN: | 2159-662X 0161-5505 |
| DOI: | 10.2967/jnumed.118.210310 |
| Popis: | In ovo studies are a valuable option in preclinical research, but imaging studies are severely limited by the costs of dedicated equipment needed for small-sized eggs. We sought to verify the feasibility of using larger, ostrich, eggs (Struthio camelus) for imaging on the PET/CT scanners used for routine clinical investigations. Methods: Ostrich eggs were incubated until shortly before hatching, prepared for intravitelline venous injection of contrast medium or radiotracer, and imaged using native CT, contrast-enhanced CT, and PET/CT. Any technical adaptations that were needed to improve the outcome were noted. Results: Of the 34 eggs initially incubated, 12 became fully available for imaging of embryonal development. In ovo imaging with conventional PET/CT not only was feasible but also provided images of good quality, including on dynamic PET imaging. Conclusion: In ovo imaging with ostrich eggs and routine clinical scanners may allow broader application of this field of preclinical research, obviating costly dedicated equipment and reducing the number of animals needed for classic animal research. Further experiments are warranted to refine this novel approach, especially to reduce motion artifacts and improve monitoring of viability. |
| Databáze: | OpenAIRE |
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