Analysis of glycoforms present in two mouse IgG2a monoclonal antibody preparations
| Autor: | JoséM. Coco-Martin, E. Coen Beuvery, Freek Brunink, Tiny A. M. van der Velden-de Groot |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Glycoside Hydrolases
medicine.drug_class Immunology Molecular Sequence Data Dot blot Monoclonal antibody Mice Lectins medicine Immunology and Allergy Animals Isoelectric Point Glycoproteins chemistry.chemical_classification biology Chemistry Lectin Antibodies Monoclonal Oligosaccharide Carbohydrate Fragment crystallizable region Molecular biology Biochemistry Carbohydrate Sequence Immunoglobulin G biology.protein Antibody Glycoprotein |
| Zdroj: | Journal of immunological methods. 155(2) |
| ISSN: | 0022-1759 |
| Popis: | Lectins have been used for the determination of the oligosaccharide structures expressed by two monoclonal IgG antibodies, MN12 and RIV6. Dot blot experiments revealed the presence of terminal Fuc alpha (1--6)GlcNAc, Gal beta (1--3)GalNAc, Gal beta (1--4)GlcNAc, Man alpha (1--6, 1--3)Man, NeuAc alpha (2--6)Gal and NeuAc alpha (2--6)GalNAc on both monoclonal antibodies. MN12 was shown to contain a carbohydrate moiety within the Fc region only. RIV6 contained carbohydrate moieties within both the Fc and Fab regions. Additional O-glycosidic linked carbohydrate chains were detected within the Fc region of both monoclonal antibodies. High mannose structures were also detected on both Mabs. |
| Databáze: | OpenAIRE |
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