Model of implantation of tumor cells simulating recurrence in colonic anastomosis in mice
| Autor: | Gideon Goldman, Yair Galili, Micha Rabau, Yoram Kluger, Arie Shnaper, Joseph Yossiphov |
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| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Pathology Colon Melanoma Experimental Rectum Adenocarcinoma Anastomosis Mice Surgical anastomosis Neoplasm Seeding Cell Movement Cytology Carcinoma medicine Animals Ascitic Fluid Mice Inbred BALB C business.industry Peritoneal fluid Anastomosis Surgical Gastroenterology General Medicine medicine.disease Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure Colonic Neoplasms Cancer cell Histopathology Neoplasm Recurrence Local business Neoplasm Transplantation |
| Zdroj: | Diseases of the Colon & Rectum. 41:1506-1510 |
| ISSN: | 0012-3706 |
| DOI: | 10.1007/bf02237297 |
| Popis: | PURPOSE: Local recurrence after colorectal cancer surgery is usually perianastomotic. An experiment was designed to investigate whether free intraluminal cells can penetrate through a colonic anastomosis and thereby cause local recurrence. METHODS: BALB/c and C57/BL mice underwent ascending colotomy followed by watertight anastomosis. Thereafter, CT-26 murine colon carcinoma cells were injected into the cecal lumen 2 cm proximal to the anastomosis of syngeneic BALB/c mice, whereas B-16 murine melanoma cells were injected in the same fashion into C57/BL mice. Control animals without anastomosis received similar injections. Animals were killed 24 hours, 72 hours, and 30 days after surgery and were checked for tumorigenesis. RESULTS: Results of peritoneal fluid cytology were negative after 24 hours, whereas after 72 hours cancer cells were identified in the peritoneal fluid of 80 percent of mice with colotomy and anastomosis compared with 20 percent of control mice. Thirty days after surgery, 11.1 percent of the control BALB/c mice developed pericecal tumor growth, similar to the overall rate of murine melanoma in C57/BL. In mice with anastomoses, perianastomotic tumor growth was observed in 47.5 percent of BALB/c mice (P |
| Databáze: | OpenAIRE |
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