Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway
| Autor: | Stefan Schillberg, Regine Eibl, Johanna Brändli, Nikolay Vasilev, Tuulikki Seppänen-Laakso, Harro J. Bouwmeester, Lemeng Dong, Nicole Imseng, Hannu Maaheimo, Sander van der Krol, Arho Virkki, Kirsi-Marja Oksman-Caldentey, Anneli Ritala, Heiko Rischer |
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| Přispěvatelé: | Publica |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
hairy root
Valeriana officinalis Geraniol DNA Plant Nicotiana tabacum Acyclic Monoterpenes Pentose Secondary Metabolism Bioengineering 660.6: Biotechnologie Biology Applied Microbiology and Biotechnology Plant Roots tobacco plastidial pathways chemistry.chemical_compound isoprenoid biosynthesis Valerian Botany Tobacco Hexose Laboratorium voor Plantenfysiologie essential oils Plant Proteins transgenic chemistry.chemical_classification Indole alkaloid Terpenes Glycoside bioreactors General Medicine biology.organism_classification Plants Genetically Modified Secologanin Tryptamine Alkaloids cultures Terpenoid Phosphoric Monoester Hydrolases key enzyme catharanthus-roseus synthase chemistry cells EPS Laboratory of Plant Physiology monoterpene Biotechnology terpenoid indole alkaloid |
| Zdroj: | Ritala, A, Dong, L, Imseng, N, Seppänen-Laakso, T, Vasilev, N, van der Krol, S, Rischer, H, Maaheimo, H, Virkki, A, Brändli, J, Schillerberg, S, Eibl, R, Bouwmeester, H & Oksman-Caldentey, K-M 2014, ' Evaluation of tobacco ( Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway ', Journal of Biotechnology, vol. 176, pp. 20-28 . https://doi.org/10.1016/j.jbiotec.2014.01.031 Journal of Biotechnology, 176, 20-28 Journal of Biotechnology 176 (2014) |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/j.jbiotec.2014.01.031 |
| Popis: | The terpenoid indole alkaloids are one of the major classes of plant-derived natural products and are well known for their many applications in the pharmaceutical, fragrance and cosmetics industries. Hairy root cultures are useful for the production of plant secondary metabolites because of their genetic and biochemical stability and their rapid growth in hormone-free media. Tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots, which do not produce geraniol naturally, were engineered to express a plastid-targeted geraniol synthase gene originally isolated from Valeriana officinalis L. (VoGES). A SPME-GC–MS screening tool was developed for the rapid evaluation of production clones. The GC–MS analysis revealed that the free geraniol content in 20 hairy root clones expressing VoGES was an average of 13.7 μg/g dry weight (DW) and a maximum of 31.3 μg/g DW. More detailed metabolic analysis revealed that geraniol derivatives were present in six major glycoside forms, namely the hexose and/or pentose conjugates of geraniol and hydroxygeraniol, resulting in total geraniol levels of up to 204.3 μg/g DW following deglycosylation. A benchtop-scale process was developed in a 20-L wave-mixed bioreactor eventually yielding hundreds of grams of biomass and milligram quantities of geraniol per cultivation bag. |
| Databáze: | OpenAIRE |
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