Quorum-sensing control of antibiotic resistance stabilizes cooperation in Chromobacterium violaceum
| Autor: | Kara C Evans, Lennel A Camuy-Vélez, Benjamin Neuenswander, Josephine R. Chandler, Saida Benomar, Xiaofei Wang, Ellen B Nasseri |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Burkholderia 030106 microbiology Population Biology Microbiology Article 03 medical and health sciences Antibiotic resistance Chromobacterium Drug Resistance Bacterial Benzopyrans education Ecology Evolution Behavior and Systematics Genetics education.field_of_study Burkholderia thailandensis fungi Quorum Sensing food and beverages biochemical phenomena metabolism and nutrition biology.organism_classification Quorum sensing Microbial Interactions bacteria Efflux Chromobacterium violaceum |
| Zdroj: | The ISME Journal. 12:1263-1272 |
| ISSN: | 1751-7370 1751-7362 |
| Popis: | Many Proteobacteria use quorum sensing to regulate production of public goods, such as antimicrobials and proteases, that are shared among members of a community. Public goods are vulnerable to exploitation by cheaters, such as quorum sensing-defective mutants. Quorum sensing- regulated private goods, goods that benefit only producing cells, can prevent the emergence of cheaters under certain growth conditions. Previously, we developed a laboratory co-culture model to investigate the importance of quorum-regulated antimicrobials during interspecies competition. In our model, Burkholderia thailandensis and Chromobacterium violaceum each use quorum sensing-controlled antimicrobials to inhibit the other species' growth. Here, we show that C. violaceum uses quorum sensing to increase resistance to bactobolin, a B. thailandensis antibiotic, by increasing transcription of a putative antibiotic efflux pump. We demonstrate conditions where C. violaceum quorum-defective cheaters emerge and show that in these conditions, bactobolin restrains cheaters. We also demonstrate that bactobolin restrains quorum-defective mutants in our co-culture model, and the increase in antimicrobial-producing cooperators drives the C. violaceum population to become more competitive. Our results describe a mechanism of cheater restraint involving quorum control of efflux pumps and demonstrate that interspecies competition can reinforce cooperative behaviors by placing constraints on quorum sensing-defective mutants. |
| Databáze: | OpenAIRE |
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