Presence of SARS-CoV-2 Viral RNA in Aqueous Humor of Asymptomatic Individuals
| Autor: | Jorge Maestre-Mesa, Daliya Dzhaber, Allen O. Eghrari, Elizabeth Fout, Ellen H. Koo, Darlene Miller, Sander R. Dubovy, Amar P Shah |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Real-Time Polymerase Chain Reaction Asymptomatic Article Cataract surgery Virus law.invention Aqueous Humor 03 medical and health sciences 0302 clinical medicine law Internal medicine medicine Humans Prospective Studies Polymerase chain reaction 030304 developmental biology 0303 health sciences Reverse Transcriptase Polymerase Chain Reaction SARS-CoV-2 business.industry Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) COVID-19 RNA Ocular manifestations Reverse transcriptase Ophthalmology Cross-Sectional Studies Nasal Swab COVID-19 Nucleic Acid Testing Aqueous humor anterior chamber Ambulatory 030221 ophthalmology & optometry RNA Viral medicine.symptom business |
| Zdroj: | American Journal of Ophthalmology |
| ISSN: | 0002-9394 |
| Popis: | Purpose The purpose of this study was to determine whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is detectable in the aqueous of asymptomatic individuals presenting for ophthalmic surgery. Design Prospective cross-sectional study. Methods Setting and participants: all patients undergoing anterior segment surgery at an ambulatory surgical center (ASC) belonging to a tertiary academic center in South Florida during a 102-day period between June and September 2020 received nasal swab testing for SARS-CoV-2 and underwent a relevant review of symptoms prior to surgery, with negative results required for both in order to proceed with surgery. Main outcomes and measurements: a small sample of aqueous humor (approximately 0.2 cc) was acquired at the beginning of anterior segment surgery from all participants. Aqueous humor was analyzed for SARS-CoV-2 viral ribonucleic acid (RNA) using real-time reverse transcriptase polymerase chain reaction. Demographic information was acquired from participants for secondary analyses. Results A total of 70 samples were acquired. Of those, 39 samples were excluded due to insufficient material or inconclusive results. Of 31 samples that were successfully analyzed, 6 (19.4%) demonstrated detectable SARS-CoV-2 RNA. None of the 6 individuals (0%) with detectable viral RNA in aqueous humor reported symptoms during the year, compared to 2 of 25 individuals (8%) with negative samples (P = 1). Positive samples were distributed throughout the study period, including both the first and the last days of enrollment. Conclusions The presence of SARS-CoV-2 viral RNA in aqueous despite negative nasal swab testing confirmed its presence beyond the blood-ocular barrier in asymptomatic individuals and raises the possibility that the virus may persist in immunoprivileged spaces despite an absence of symptoms. |
| Databáze: | OpenAIRE |
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