DELAY OF GERMINATION 1-LIKE 4 acts as an inducer of seed reserve accumulation

Autor: Bas J.W. Dekkers, Mariko Nonogaki, David A. Hendrix, Ryosuke Koyari, Leo A. J. Willems, Khadidiatou Sall, Leónie Bentsink, Hiroyuki Nonogaki, Yoshihiko Katsuragawa
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
dormancy
hormone
seed maturation
Mutant
Arabidopsis
Germination
Plant Science
Biology
01 natural sciences
abscisic acid
03 medical and health sciences
chemistry.chemical_compound
Plant Growth Regulators
Gene Expression Regulation
Plant
Genetics
Storage protein
Laboratorium voor Plantenfysiologie
Gene
Abscisic acid
Phylogeny
chemistry.chemical_classification
Arabidopsis Proteins
Gene Expression Profiling
Seed dormancy
food and beverages
Gene Expression Regulation
Developmental
Cell Biology
Plants
Genetically Modified
Cell biology
DNA-Binding Proteins
030104 developmental biology
Phenotype
storage proteins
chemistry
Seeds
Dormancy
EPS
Oleosin
Laboratory of Plant Physiology
seed development
010606 plant biology & botany
Abscisic Acid
Transcription Factors
Zdroj: Plant Journal, 100(1), 7-19
Plant Journal 100 (2019) 1
ISSN: 1365-313X
0960-7412
Popis: More than 70% of global food supply depends on seeds. The major seed reserves, such as proteins, lipids, and polysaccharides, are produced during seed maturation. Here, we report that DELAY OF GERMINATION 1-LIKE 4 (DOGL4) is a major inducer of reserve accumulation during seed maturation. The DOGL family proteins are plant-specific proteins of largely unknown biochemical function. DOGL4 shares only limited homology in amino acid sequence with DOG1, a major regulator of seed dormancy. DOGL4 was identified as one of the outstanding abscisic acid (ABA)-induced genes in our RNA sequencing analysis, whereas DOG1 was not induced by ABA. Induction of DOGL4 caused the expression of 70 seed maturation-specific genes, even in germinating seeds, including the major seed reserves ALBUMIN, CRUCIFERIN and OLEOSIN. Although DOG1 affects the expression of many seed maturation genes, the major seed reserve genes induced by DOGL4 are not altered by the dog1 mutation. Furthermore, the reduced dormancy and longevity phenotypes observed in the dog1 seeds were not observed in the dogl4 mutants, suggesting that these two genes have limited functional overlap. Taken together, these results suggest that DOGL4 is a central factor mediating reserve accumulation in seeds, and that the two DOG1 family proteins have diverged over the course of evolution into independent regulators of seed maturation, but retain some overlapping function.
Databáze: OpenAIRE
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