A study of the relationship between nuclear contour thickening, nuclear enlargement and human papillomavirus infection in squamous cells

Autor:	Junko Iijima, Mitsuaki Okodo, Koji Teruya, Kaori Okayama, Natsuko Shiina, Hiromi Yabusaki, Hiromi Hata
Rok vydání:	2019
Předmět:	Pathology medicine.medical_specialty Histology Uterine Cervical Neoplasms Papanicolaou stain 030209 endocrinology & metabolism Malignancy Pathology and Forensic Medicine Lesion 03 medical and health sciences 0302 clinical medicine Pregnancy Atypical Squamous Cells of the Cervix Humans Medicine Papillomaviridae Cervix business.industry Papillomavirus Infections HPV infection Epithelial Cells General Medicine Odds ratio Uterine Cervical Dysplasia medicine.disease Confidence interval medicine.anatomical_structure Colposcopy 030220 oncology & carcinogenesis Female medicine.symptom business Oncovirus Papanicolaou Test
Zdroj:	Cytopathology. 30:644-649
ISSN:	1365-2303 0956-5507
DOI:	10.1111/cyt.12756
Popis:	Objective To investigate the relationship between the morphological features of nuclear enlarged cells and human papillomavirus (HPV) infection in negative for intraepithelial lesion or malignancy (NILM) and atypical squamous cells of undetermined significance (ASC-US). Methods In total, 128 Papanicolaou specimens comprising 41 ASC-US cases and 87 NILM cases were examined. Cell morphological analysis was performed using both area ratio (nuclear area in cells with nuclear enlargement/nuclear area in normal intermediate cells) and nuclear contour thickening. High-risk HPV was detected using the Uniplex E6/E7 polymerase chain reaction assay and logistic regression analyses of factors related to high-risk HPV infection were performed. Results Nuclear contour thickening was present in 57.7% (64/111 cells) of high-risk HPV positive cases and 21.8% (69/317 cells) of high-risk HPV negative cases. There was a statistically significant association (P = 0.01) between high-risk HPV infection and nuclear contour thickening. Nuclear contour thickening was approximately one-third higher in NILM cases than in ASC-US cases (odds ratio, 0.371; 95% confidence interval, 0.208-0.662) and three times higher in high-risk HPV-positive cases than in high-risk HPV-negative cases (odds ratio, 2.831; 95% confidence interval, 1.591-5.039). Conclusion Our results suggest that nuclear contour thickening in nuclear enlarged cells in NILM and ASC-US cases may be a cellular finding associated with HPV infection.
Databáze:	OpenAIRE
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