Phenotypic and genomic diversity of Lactobacillus plantarum strains isolated from various environmental niches
| Autor: | Michiel Kleerebezem, Johan E. T. van Hylckama Vlieg, Vesela A. Tzeneva, Hoa T. K. Phan, Douwe Molenaar, Marjo J. C. Starrenburg, J. L. W. Rademaker, Roland J. Siezen, Michiel Wels, Anna Castioni |
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| Přispěvatelé: | Molecular Cell Physiology, AIMMS |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
DNA
Bacterial Energy and redox metabolism [NCMLS 4] Molecular Sequence Data Biology Subspecies Environment Lactobacillus plantarum diversità intraspecifica microarray Genome Microbiology pcr Microbiologie RNA Ribosomal 16S evolution paraplantarum Animals Cluster Analysis Humans Gene Ecology Evolution Behavior and Systematics streptococcus-thermophilus Genetics Ecological niche lactic-acid bacteria Genetic diversity WIMEK horizontal gene-transfer starter cultures food and beverages Biodiversity differentiation sequence biology.organism_classification Phenotype bacteria identification Niche adaptation Genome Bacterial Reference genome |
| Zdroj: | Environmental Microbiology 12 (2010) 3 Environmental Microbiology, 12(3), 758-773 Environmental Microbiology, 12, 3, pp. 758-73 Environmental Microbiology, 12(3), 758-773. Wiley-Blackwell Siezen, R J, Tzeneva, V A, Castioni, A, Wels, M, Phan, H T K, Rademaker, J L W, Starrenburg, M J C, Kleerebezem, M, Molenaar, D & van Hylckama Vlieg, J E T 2010, ' Phenotypic and genomic diversity of Lactobacillues plantarum strains isolated from various environmental niches. ', Environmental Microbiology, vol. 12, no. 3, pp. 758-773 . https://doi.org/10.1111/j.1462-2920.2009.02119.x Environmental Microbiology, 12, 758-73 |
| ISSN: | 1462-2912 |
| Popis: | Item does not contain fulltext Lactobacillus plantarum is a ubiquitous microorganism that is able to colonize several ecological niches, including vegetables, meat, dairy substrates and the gastro-intestinal tract. An extensive phenotypic and genomic diversity analysis was conducted to elucidate the molecular basis of the high flexibility and versatility of this species. First, 185 isolates from diverse environments were phenotypically characterized by evaluating their fermentation and growth characteristics. Strains clustered largely together within their particular food niche, but human fecal isolates were scattered throughout the food clusters, suggesting that they originate from the food eaten by the individuals. Based on distinct phenotypic profiles, 24 strains were selected and, together with a further 18 strains from an earlier low-resolution study, their genomic diversity was evaluated by comparative genome hybridization against the reference genome of L. plantarum WCFS1. Over 2000 genes were identified that constitute the core genome of the L. plantarum species, including 121 unique L. plantarum-marker genes that have not been found in other lactic acid bacteria. Over 50 genes unique for the reference strain WCFS1 were identified that were absent in the other L. plantarum strains. Strains of the L. plantarum subspecies argentoratensis were found to lack a common set of 24 genes, organized in seven gene clusters/operons, supporting their classification as a separate subspecies. The results provide a detailed view on phenotypic and genomic diversity of L. plantarum and lead to a better comprehension of niche adaptation and functionality of the organism. 01 maart 2010 |
| Databáze: | OpenAIRE |
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