NCR1+ cells appear early in GALT development of the ovine foetus and acquire a c-kit+ phenotype towards the end of gestation
| Autor: | Line Olsen, Preben Boysen, Mona Aleksandersen, Caroline Piercey Åkesson, Charles McL. Press, Arild Espenes, Françoise Drouet, Anne K. Storset |
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| Přispěvatelé: | Norwegian University of Life Sciences (NMBU), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Department of Food Safety & Infection Biology, Faculty of Veterinary Medicine and Biosciences, Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
TISSUES Lymphoid Tissue CD79 T cell CD3 Immunology CD34 INNATE LYMPHOID-CELLS Innate lymphoid cells NK cells DENDRITIC CELLS FETAL LAMBS 03 medical and health sciences NATURAL-KILLER-CELLS Intestinal mucosa medicine Animals GALT Lymphocytes Intestinal Mucosa LEUKOCYTE POPULATIONS General Veterinary biology Natural Cytotoxicity Triggering Receptor 1 [SDV.BA]Life Sciences [q-bio]/Animal biology Innate lymphoid cell Foetus ONTOGENY NCR1 ILEAL PEYERS PATCH Molecular biology Lymphocyte Subsets Ovine Intestines NK-CELLS Proto-Oncogene Proteins c-kit Phenotype 030104 developmental biology medicine.anatomical_structure Lymphatic system SHEEP biology.protein Antibody |
| Zdroj: | Veterinary Immunology and Immunopathology Veterinary Immunology and Immunopathology, Elsevier, 2016, 169, pp.79-84. ⟨10.1016/j.vetimm.2015.12.008⟩ |
| ISSN: | 0165-2427 |
| DOI: | 10.1016/j.vetimm.2015.12.008⟩ |
| Popis: | International audience; The amount, distribution and phenotype of ovine NCR1+ cells were investigated during developing GALT from day 70 of gestation. Antibodies against CD3 and CD79 were used to identify the compartments of GALT, and the localization of NCR1+ cells were correlated within these structures. Markers CD34 and c-kit, in addition to Ki67, were used to investigate possible origin and the stage of development of the NCR1+ cells. NCR1+ cells were present as single cells in the subepithelial tissue as early as 70 days of gestation, and were predominantly present in the T cell rich IFAs and domes as these intestinal wall compartments developed. While NCR1+ cells proliferated more intensively at mid-gestation (70-104 days), the number of NCR1+ cells also expressing c-kit, increased at the end of gestation. In conclusion, NCR1+ cells appeared early in T cell areas of the gut and displayed a phenotype consistent with intermediate stages of cNK cells and/or a subpopulation of ILC22. (C) 2016 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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