The 24-Angstrom Structure of Respiratory Syncytial Virus Nucleocapsid Protein-RNA Decameric Rings
| Autor: | Colin Loney, David Bhella, Kirsty MacLellan, R. Paul Yeo |
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| Rok vydání: | 2007 |
| Předmět: |
Models
Molecular Macromolecular Substances viruses Immunology medicine.disease_cause Microbiology Vesicular stomatitis Indiana virus Virus law.invention Microscopy Electron Transmission law Virology Image Processing Computer-Assisted medicine Mononegavirales biology Structure and Assembly Rabies virus Resolution (electron density) RNA Nucleocapsid Proteins biology.organism_classification Negative stain Respiratory Syncytial Viruses Vesicular stomatitis virus Insect Science RNA Viral Electron microscope |
| Zdroj: | Journal of Virology. 81:9519-9524 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.00526-07 |
| Popis: | Respiratory syncytial virus (RSV), a nonsegmented, negative-sense RNA-containing virus, is a common cause of lower respiratory tract disease. Expression of RSV nucleocapsid protein (N) in insect cells using the baculovirus expression system leads to the formation of N-RNA complexes that are morphologically indistinguishable from viral nucleocapsids. When imaged in an electron microscope, three distinct types of structures were observed: tightly wound short-pitch helices, highly extended helices, and rings. Negative stain images of N-RNA rings were used to calculate a three-dimensional reconstruction at 24 Å resolution, revealing features similar to those observed in nucleocapsids from other viruses of the order Mononegavirales . The reconstructed N-RNA rings comprise 10 N monomers and have an external radius of 83 Å and an internal radius of 40 Å. Comparison of this structure with crystallographic data from rabies virus and vesicular stomatitis virus N-RNA rings reveals striking morphological similarities. |
| Databáze: | OpenAIRE |
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