Inhibitory regulation of purple sweet potato polysaccharide on the hepatotoxicity of tri-(2,3-dibromopropyl) isocyanate
| Autor: | Furui Han, Liangliang Zhao, Zheng Qi, Ming Yang, Zhiwei Le, Yajie Feng, Chenfeng Ji |
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| Rok vydání: | 2022 |
| Předmět: |
Liver injury
Antioxidant medicine.diagnostic_test Chemistry medicine.medical_treatment Environmental pollution Hep G2 Cells General Medicine Mitochondrion medicine.disease Biochemistry Western blot Polysaccharides Structural Biology In vivo Apoptosis Dietary Carbohydrates medicine Humans MTT assay Chemical and Drug Induced Liver Injury Ipomoea batatas Molecular Biology Isocyanates |
| Zdroj: | International Journal of Biological Macromolecules. 194:445-451 |
| ISSN: | 0141-8130 |
| Popis: | Tri-(2,3-dibromopropyl) isocyanate (TBC), a new emerged persistent organic pollutant, is widely used in fields of flame retardant, textile, rubber and plastic with strong hepatotoxicity. Purple Sweet Potato Polysaccharide (PSPP) has antioxidant and hepatoprotective effects. This study aims to answer the scientific question whether PSPP has a protective effect on TBC induced liver injury. The effect of PSPP on the apoptosis of HepG2 cells was detected by MTT assay, the morphological changes were observed by morphological observation, and the apoptosis rate was determined by flow cytometry. The apoptotic genes were detected by qPCR assay, the relevant protein express was detected by western blot. The correlation between proteins and genes in the apoptosis pathway of HepG2 cells was calculated. To further reveal the apoptosis mechanism of TBC hepatotoxicity in vivo, 19 target genes and 14 apoptotic related proteins of inhibiting apoptosis via death receptor and mitochondria were discussed, all the above results proved that PSPP had protective effect on liver injury induced by TBC. This study not only provided a scientific basis for clarifying the mechanism of TBC hepatotoxicity and the protective effect of PSPP, but also generated the new point and method in terms of the prevention in advance and early intervention of diseases caused by environmental pollution. |
| Databáze: | OpenAIRE |
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