Establishment of an experimental model for MHC homo-to-hetero transplantation
| Autor: | Tomoki Murata, Hyuma Tsuji, Nanami Eguchi, Airi Sasaki, Ken-ichiro Seino, Ryo Otsuka, Haruka Wada, Tatsuo Kawai, Mizuho Itoh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Graft Rejection
Male 0301 basic medicine T cell Transplantation Heterologous T cells lcsh:Medicine Heterologous Major histocompatibility complex Article Mice 03 medical and health sciences 0302 clinical medicine Immune system Isoantibodies Histocompatibility Antigens Homologous chromosome medicine Animals Transplantation Homologous lcsh:Science Induced pluripotent stem cell Mice Inbred BALB C Multidisciplinary biology business.industry Graft Survival lcsh:R Allotransplantation Skin Transplantation Models Theoretical Tissue Donors Mice Inbred C57BL Transplantation Induced pluripotent stem cells surgical procedures operative 030104 developmental biology medicine.anatomical_structure Immunology Mice Inbred CBA biology.protein Female lcsh:Q MHC Antibody business 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Preventing rejection is a major challenge in transplantation medicine, even when using pluripotent stem cell-derived grafts. In iPS cell (iPSC)-based transplantation, to reduce the risk of rejection, it is thought to be optimal that preparing the cells from donors whose human leukocyte antigen-haplotype are homozygous. Generally, this approach is referred to as major histocompatibility complex (MHC) homo-to-hetero transplantation, which is MHC-matched but minor antigen-mismatched. To investigate the immune response in the MHC homo-to-hetero transplantation, we established a murine experimental system in which MHC-matched but minor antigen-mismatched tissue (skin) grafts were transplanted into MHC-heterozygous recipients. Unexpectedly, only minor antigen-mismatched grafts were rejected at the same time points as rejection of fully allogeneic grafts. A vigorous anti-donor type T cell response was detected in vitro and conventional immunosuppressants targeting T cell activation had limited effects on controlling rejection. However, anti-donor antibodies were not detected only in the minor antigen-mismatched transplantation. This murine transplantation model can be used to further analyze immunological subjects for MHC homo-to-hetero iPSC-based transplantation. |
| Databáze: | OpenAIRE |
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