Sensitive and Simultaneous Detection of SARS-CoV-2-Specific IgM/IgG Using Lateral Flow Immunoassay Based on Dual-Mode Quantum Dot Nanobeads
| Autor: | Luoluo Shi, Shengqi Wang, Haifeng Liu, Xingsheng Yang, Xiaodan Cheng, Zihui Zhou, Bing Gu, Chongwen Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
viruses
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 010402 general chemistry medicine.disease_cause Antibodies Viral 01 natural sciences Sensitivity and Specificity Virus Immunoglobulin G Article Analytical Chemistry Quantum Dots medicine Humans Particle Size Coronavirus Immunoassay biology medicine.diagnostic_test Chemistry SARS-CoV-2 010401 analytical chemistry virus diseases COVID-19 Silicon Dioxide Virology 0104 chemical sciences Immunoglobulin M Quantum dot Spike Glycoprotein Coronavirus biology.protein Gold Antibody |
| Zdroj: | Analytical Chemistry |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.0c03484 |
| Popis: | A rapid and accurate method for detection of virus (SARS-CoV-2)-specific antibodies is important to contain the 2019 coronavirus disease (COVID-19) outbreak, which is still urgently needed. Here, we develop a colorimetric-fluorescent dual-mode lateral flow immunoassay (LFIA) biosensor for rapid, sensitive, and simultaneous detection of SARS-CoV-2-specific IgM and IgG in human serum using spike (S) protein-conjugated SiO2@Au@QD nanobeads (NBs) as labels. The assay only needs 1 μL of the serum sample, can be completed within 15 min, and is 100 times more sensitive than the colloidal gold-based LFIA. Two detection modes of our biosensor are available: the colorimetric mode for rapid screening of the patients with suspected SARS-CoV-2 infection without any special instrument and the fluorescent mode for sensitive and quantitative analyses to determine the concentrations of specific IgM/IgG in human serum and detect the infection early and precisely. We validated the proposed method using 16 positive serum samples from patients with COVID-19 and 41 negative samples from patients with other viral respiratory infections. The results demonstrated that combined detection of virus-specific IgM and IgG via SiO2@Au@QD LFIA can identify 100% of patients with SARS-CoV-2 infection with 100% specificity. |
| Databáze: | OpenAIRE |
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