Structural elucidation and immuno-stimulatory activity of a novel polysaccharide containing glucuronic acid from the fungus Echinodontium tinctorium
| Autor: | Christian Heiss, Parastoo Azadi, Mehreen Zeb, Chow H. Lee, Grace Lu, Kerry B. Reimer, Keith N. Egger, Linda E. Tackaberry, Hugues B. Massicotte |
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| Rok vydání: | 2021 |
| Předmět: |
Arabinose
Magnetic Resonance Spectroscopy Polymers and Plastics Rhamnose Stereochemistry 02 engineering and technology 010402 general chemistry Polysaccharide Methylation 01 natural sciences Gas Chromatography-Mass Spectrometry Fucose Mice chemistry.chemical_compound Glucuronic Acid Polysaccharides Spectroscopy Fourier Transform Infrared Materials Chemistry Animals chemistry.chemical_classification Xylose biology Basidiomycota Macrophages Monosaccharides Organic Chemistry Galactose Glycosidic bond 021001 nanoscience & nanotechnology biology.organism_classification Glucuronic acid 0104 chemical sciences Glucose RAW 264.7 Cells chemistry Echinodontium tinctorium Cytokines Chemokines 0210 nano-technology Mannose |
| Zdroj: | Carbohydrate Polymers. 258:117700 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2021.117700 |
| Popis: | An immuno-stimulatory polysaccharide (EtISPFa) was purified from water extract of the fungus Echinodontium tinctorium. EtISPFa has an estimated weight average molecular weight (Mw) of 1354 kDa and is composed of glucose (66.2 %), glucuronic acid (10.1 %), mannose (6.7 %), galactose (6.4 %), xylose (5.6 %), rhamnose (3.1 %), fucose (1.8 %), and arabinose (0.2 %). It has multiple glycosidic linkages, with 3-Glcp (19.8 %), 4-GlcpA (10.8 %), 6-Glcp (10.7 %), and 3,6-Glcp (8.7 %) being the most prominent. NMR analysis showed that EtISPFa has a backbone containing mostly of 3-substituted β-glucopyranose with 4-substituted glucopyranosyluronic acid. Short side chains consisting of an average of two β-glycopyranose residues, connected through 1→6 linkages, are attached to the 6-position of about every 4th or 5th backbone glucose residue. EtISPFa is a novel glucuronic acid-containing β-glucan capable of significantly inducing the production of cytokines IL-17, IL-16, MIP-2, G-CSF,GM-CSF, LIF, MIP-1α, MIP-1β, and RANTES in vitro. EtISPFa should be further explored for its immuno-stimulatory activity in vivo. |
| Databáze: | OpenAIRE |
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