Antimicrobial effect of a modified vanadium chloroperoxidase on Enterococcus faecalis biofilms at root canal pH
| Autor: | Aloysius F. Hartog, Paul R. Wesselink, Michel A. Hoogenkamp, Aleksandra Bury, I.F. Persoon, Wim Crielaard, Ron Wever |
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| Přispěvatelé: | Endodontology, Preventive Dentistry, Endodontologie (OII, ACTA), Biocatalysis (HIMS, FNWI), Preventieve tandheelkunde (OII, ACTA) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Bromides
Hypohalite Time Factors Materials science Biocompatibility Sodium Hypochlorite Root canal Biocompatible Materials Enterococcus faecalis Microbiology chemistry.chemical_compound Materials Testing medicine Humans Hydrogen peroxide General Dentistry chemistry.chemical_classification Root Canal Irrigants biology Cyclohexanones Mouth Mucosa Biofilm Hydrogen Peroxide Fibroblasts Hydrogen-Ion Concentration Oxidants Antimicrobial biology.organism_classification Anti-Bacterial Agents Enzyme medicine.anatomical_structure chemistry Biofilms Dental Pulp Cavity Chloride Peroxidase |
| Zdroj: | Persoon, I F, Hoogenkamp, M A, Bury, A, Wesselink, P R, Hartog, A F, Wever, R & Crielaard, W 2013, ' Antimicrobial effect of a modified vanadium chloroperoxidase on Enterococcus faecalis biofilms at root canal pH ', The Journal of Endodontics, vol. 39, no. 8, pp. 1035-1038 . https://doi.org/10.1016/j.joen.2013.04.038 The Journal of Endodontics, 39(8), 1035-1038. Elsevier Inc. The Journal of Endodontics, 39(8), 1035-1038. Elsevier |
| ISSN: | 0099-2399 |
| DOI: | 10.1016/j.joen.2013.04.038 |
| Popis: | IntroductionPrevious research showed an antimicrobial effect of vanadium chloroperoxidase (VCPO) on in vitro Enterococcus faecalis biofilms. The current study aimed to optimize the use of this enzyme at the root canal pH using a modified VCPO (mVCPO) that was adapted to function at a higher pH and to explore the biocompatibility of mVCPO.MethodsThe activity of the original and modified VCPO was assessed using the monochlorodimedone assay. For antimicrobial assessment, 48-hour biofilms of E. faecalis OS-16 were incubated 5 or 30 minutes with mVCPO, bromide, and hydrogen peroxide, and colony-forming units were determined. A metabolic activity assay was used to evaluate the cytotoxic effect of mVCPO on oral fibroblasts.ResultsReaction products generated by mVCPO at a root canal pH of 7.7 significantly inactivated the biofilm after 5 minutes and even more after 30 minutes (Mann-Whitney U test, P < .05). The mVCPO reaction products showed less cytotoxic effects than control solutions and 0.5% sodium hypochlorite (Kruskal-Wallis test, P < .05).ConclusionsThe incubation of mVCPO in the presence of its substrates with in vitro E. faecalis biofilms showed a significant antimicrobial effect at the root canal pH. Also, cytotoxicity tests showed preliminary biocompatibility. Therefore, an interappointment dressing containing mVCPO could aid in improving current endodontic treatment through continuous and local generation of antimicrobials. |
| Databáze: | OpenAIRE |
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