The distribution of pituitary adenylate cyclase-activating polypeptide-like immunoreactivity is distinct from helodermin- and helospectin-like immunoreactivities in the rat brain
| Autor: | A. Absood, Frank Sundler, L. Kivipelto, Rolf Håkanson, Akira Arimura, Pertti Panula |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Cerebellum Radioimmunoassay Hippocampus Neuropeptide Biology Supraoptic nucleus 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Internal medicine medicine Animals 030304 developmental biology 0303 health sciences Neuropeptides Brain Rats Inbred Strains Immunohistochemistry Rats medicine.anatomical_structure Endocrinology nervous system Hypothalamus Cerebral cortex Median eminence Intercellular Signaling Peptides and Proteins Pituitary Adenylate Cyclase-Activating Polypeptide Peptides Nucleus hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery |
| Zdroj: | Journal of Chemical Neuroanatomy. 5:85-94 |
| ISSN: | 0891-0618 |
| DOI: | 10.1016/0891-0618(92)90036-p |
| Popis: | Pituitary adenylate cyclase-activating polypeptide (PACAP) is an amidated 38-residue polypeptide isolated from the ovine hypothalamus. Helodermin, a 35-amino acid peptide, and helospectins, peptides of 38 and 37 amino acid residues, have been isolated from lizard venom. PACAP, helodermin and helospectins share structural features and have a similar profile of pharmacological effects: they stimulate adenylate cyclase. We studied the distribution and characteristics of PACAP-like immunoreactivity in the rat brain with immunochemical and immunohistochemical methods and compared its distribution with that of helodermin- and helospectin-like immunoreactivities. With radioimmunoassay, the highest concentrations of PACAP-like immunoreactivity were found in the hypothalamus and cerebellum. PACAP-immunoreactive cell bodies were located immunohistochemically in the supraoptic nucleus, paraventricular and periventricular hypothalamic nuclei, and in the central grey. PACAP-immunoreactive fibres and terminals were detected in the medial part of the central nucleus of amygdala, in the median eminence and neurohypophysis, and in the central grey. No PACAP-immunoreactive structures were observed in areas such as the cerebral cortex, hippocampus, or cerebellum. The distribution of PACAP-like immunoreactivity differed considerably from the distribution of helodermin- and helospectin-like immunoreactivities. The results of this study suggest that PACAP is a neuropeptide with a role in the regulation of endocrine function in the hypothalamo-hypophyseal axis. |
| Databáze: | OpenAIRE |
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