Application of thermoreversible hydrogel (poloxamer 407) to protect the corneal endothelium during phacoemulsification in porcine and rabbit eyes
| Autor: | Chong Su Cho, Jung Yeol Choi, Young Keun Han |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Corneal endothelium
medicine.medical_specialty Time Factors genetic structures Endothelium Anterior Chamber Swine medicine.medical_treatment Sodium hyaluronate Cell Count Poloxamer 02 engineering and technology Body Temperature Surface-Active Agents 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lens Implantation Intraocular Ophthalmology Hyaluronic acid medicine Animals Phacoemulsification Chemistry Endothelium Corneal Corneal Endothelial Cell Loss 021001 nanoscience & nanotechnology eye diseases Sensory Systems medicine.anatomical_structure Poloxamer 407 Self-healing hydrogels 030221 ophthalmology & optometry Surgery Rabbits 0210 nano-technology medicine.drug |
| Zdroj: | Journal of Cataract and Refractive Surgery. 44:1254-1260 |
| ISSN: | 0886-3350 |
| DOI: | 10.1016/j.jcrs.2018.04.043 |
| Popis: | To evaluate the effectiveness of thermoreversible (poloxamer) hydrogels as a substitute for ophthalmic viscosurgical devices (OVDs) during phacoemulsification in porcine and rabbit eyes and compare their endothelial protective effect with that of hyaluronic acid-based OVDs.Department of Ophthalmology, Seoul Metropolitan Government-Seoul National University Boramae Medical Center, Seoul, South Korea.Experimental study.Fluorescein-stained poloxamer hydrogels (20%, 22%, 24%, and 26% [weight/weight%]) and cohesive (sodium hyaluronate 1.0% [Provisc]) and dispersive (sodium hyaluronate 3.0%-chondroitin sulfate 4.0% [Viscoat]) OVDs were injected into the anterior chamber of porcine eyes incubated at 32°C. In the in vitro study, the retention time was measured in 3 groups of 45 porcine eyes during continuous phacoemulsification. In the in vivo study, the endothelial cell count (ECC) was measured before and 3 days after intermittent phacoemulsification in 12 rabbit eyes randomized to a poloxamer hydrogel or a dispersive OVD group.The optimum concentration of thermosensitive hydrogel was 26%, at which no gel-to-sol phase transition occurred in the anterior chamber, with a 21°C irrigation solution. In the in vitro study, the mean retention times were 5.53 seconds ± 1.77 (SD), 125.00 ± 29.34 seconds, and 221.53 ± 42.48 seconds in the cohesive OVD, dispersive OVD, and 26% poloxamer hydrogel groups, respectively (P .001). Throughout the 5-minute intermittent phacoemulsification, the 26% poloxamer hydrogel remained in the anterior chamber as a semisolid gel. In the in vivo study, the mean decrease in ECC was significantly lower in the 26% poloxamer hydrogel group than in the dispersive OVD group (P = .029).Thermoreversible hydrogels might be suitable substitutes for hyaluronic acid-based OVDs for corneal endothelial protection during phacoemulsification. |
| Databáze: | OpenAIRE |
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