The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins

Autor: Brigitte Bathe, Iris Wiegräbe, Alice C. McHardy, Nicole Dusch, Volker F. Wendisch, Jörn Kalinowski, Lothar Eggeling, Bettina Möckel, Reinhard Krämer, Bernhard J. Eikmanns, Lars Gaigalat, Klaus Huthmacher, Andreas Tauch, Oliver Rupp, Michael Hartmann, Daniel Rey, Nicole Bischoff, Daniela Bartels, Alexander Goesmann, Christian Rückert, Folker Meyer, Alfred Pühler, Hermann Sahm, Andreas Burkovski, Walter Pfefferle, Michael Bott, Burkhard Linke
Rok vydání: 2003
Předmět:
pantothenate overproduction
Proteome
Sequence analysis
Base pair
coryneform bacteria
vitamin production
genome sequence
Molecular Sequence Data
Bioengineering
Biology
Corynebacterium
Applied Microbiology and Biotechnology
Genome
nucleotide-sequence
l-isoleucine
Corynebacterium glutamicum
Multienzyme Complexes
Sequence Homology
Nucleic Acid
Databases
Genetic
biochemical-characterization
phosphotransferase system
molecular analysis
Amino Acid Sequence
Amino Acids
fine-structural analysis
Gene
Peptide sequence
Whole genome sequencing
Genetics
Aspartic Acid
Base Sequence
amino acid production
Gene Expression Profiling
Nucleic acid sequence
bioinformatics
General Medicine
Sequence Analysis
DNA
Vitamins
Recombinant Proteins
Biochemistry
escherichia-coli
bacteria
brevibacterium-flavum
corynebacterium glutamicum
Genome
Bacterial
Biotechnology
Zdroj: Journal of biotechnology. 104(1-3)
ISSN: 0168-1656
Popis: The complete genomic sequence of Corynebacterium glutainicum ATCC 13032, well-known in industry for the production of amino acids, e.g. of L-glutamate and L-lysine was determined. The C glutamician genome was found to consist of a single circular chromosome comprising 3 282 708 base pairs. Several DNA regions of unusual composition were identified that were potentially acquired by horizontal gene transfer, e.g. a segment of DNA from C diphtheriae and a prophage-containing region. After automated and manual annotation, 3002 protein-coding genes have been identified, and to 2489 of these, functions were assigned by homologies to known proteins. These analyses confirm the taxonomic position of C glutamicum as related to Mycobacteria and show a broad metabolic diversity as expected for a bacterium living in the soil. As an example for biotechnological application the complete genome sequence was used to reconstruct the metabolic flow of carbon into a number of industrially important products derived from the amino acid L-aspartate. (C) 2003 Elsevier B.V. All rights reserved.
Databáze: OpenAIRE
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