Skeletal muscle cell contraction reduces a novel myokine, chemokine (C-X-C motif) ligand 10 (CXCL10): potential roles in exercise-regulated angiogenesis
| Autor: | Keitaro Yamanouchi, Takashi Matsuwaki, Yuri Ishiuchi, Kazuki Tsujimura, Hitoshi Sato, Hideo Kawaguchi, Taku Nedachi, Masugi Nishihara |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chemokine MAP Kinase Signaling System Angiogenesis Muscle Fibers Skeletal Gene Expression Enzyme-Linked Immunosorbent Assay Polymerase Chain Reaction Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Mice 03 medical and health sciences Physical Conditioning Animal Myokine medicine Animals CXCL10 Molecular Biology biology Chemistry Myogenesis Organic Chemistry Skeletal muscle General Medicine Cell biology Chemokine CXCL10 Endothelial stem cell 030104 developmental biology medicine.anatomical_structure Exercise Test biology.protein Angiogenesis Inducing Agents C2C12 Muscle Contraction Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 82:97-105 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1080/09168451.2017.1411778 |
| Popis: | Accumulating evidence indicates that skeletal muscle secrets proteins referred to as myokines and that exercise contributes to their regulation. In this study, we propose that chemokine (C-X-C motif) ligand 10 (CXCL10) functions as a novel myokine. Initially, we stimulated differentiated C2C12 myotubes with or without electrical pulse stimulation (EPS) to identify novel myokines. Cytokine array analysis revealed that CXCL10 secretion was significantly reduced by EPS, which was further confirmed by enzyme-linked immunosorbent assay and quantitative polymerase chain reaction analysis. Treadmill experiments in mice identified significant reduction of Cxcl10 gene expression in the soleus muscle. Additionally, contraction-dependent p38 MAPK activation appeared to be involved in this reduction. Furthermore, C2C12 conditioned medium obtained after applying EPS could induce survival of MSS31, a vascular endothelial cell model, which was partially attenuated by the addition of recombinant CXCL10. Overall, our findings suggest CXCL10 as a novel exercise-reducible myokine, to control endothelial cell viability. CXCL10 as a novel exercise-reducible myokine. |
| Databáze: | OpenAIRE |
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