Large-Scale functionalized metasurface-based SARS-CoV-2 detection and quantification
| Autor: | Rajib Ahmed, Carlos F. Guimarães, Jie Wang, Fernando Soto, Asma H. Karim, Zhaowei Zhang, Rui L. Reis, Demir Akin, Ramasamy Paulmurugan, Utkan Demirci |
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| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Science & Technology
SARS-CoV-2 Microfluidics Engenharia e Tecnologia::Outras Engenharias e Tecnologias General Engineering General Physics and Astronomy Proteins Point-of-care detection COVID-19 Biosensing Techniques Metals Surface plasmon resonance Humans Fano resonance General Materials Science Large-scale metasurface |
| Popis: | Plasmonic metasurfaces consist of metal–dielectric interfaces that are excitable at background and leakage resonant modes. The sharp and plasmonic excitation profile of metal-free electrons on metasurfaces at the nanoscale can be used for practical applications in diverse fields, including optoelectronics, energy harvesting, and biosensing. Currently, Fano resonant metasurface fabrication processes for biosensor applications are costly, need clean room access, and involve limited small-scale surface areas that are not easy for accurate sample placement. Here, we leverage the large-scale active area with uniform surface patterns present on optical disc-based metasurfaces as a cost-effective method to excite asymmetric plasmonic modes, enabling tunable optical Fano resonance interfacing with a microfluidic channel for multiple target detection in the visible wavelength range. We engineered plasmonic metasurfaces for biosensing through efficient layer-by-layer surface functionalization toward real-time measurement of target binding at the molecular scale. Further, we demonstrated the quantitative detection of antibodies, proteins, and the whole viral particles of SARS-CoV-2 with a high sensitivity and specificity, even distinguishing it from similar RNA viruses such as influenza and MERS. This cost-effective plasmonic metasurface platform offers a small-scale light-manipulation system, presenting considerable potential for fast, real-time detection of SARS-CoV-2 and pathogens in resource-limited settings Stanford RISE COVID-19 Crisis Response Faculty Seed Grant Program and Stanford PHIND Seed Grant Program. C.F.G. acknowledges support from Fundação para a Ciência e Tecnologia (Grant No. PD/BD/135253/2017) as well as Fundação LusoAmericana Para o Desenvolvimento (FLAD). F.S. was supported by the Schmidt Science Fellows in partnership with the Rhodes Trust. U.D. is a founder of and has an equity interest in (i) DxNow Inc., a company that is developing microfluidic IVF tools and imaging technologies, (ii) Koek Biotech, a company that is developing microfluidic technologies for clinical solutions, (iii) Levitas Inc., a company focusing on developing microfluidic sorters using magnetic levitation, (iv) Hillel Inc. and Hillal Biotech companies, bringing microfluidic cell phone tools to home settings, and (v) Mercury Biosciences, a company developing vesicle isolation technologies. U.D.’s interests were viewed and managed in accordance with the conflict-of-interest policies of Stanford University |
| Databáze: | OpenAIRE |
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