Direct contact between dendritic cells and bronchial epithelial cells inhibits T cell recall responses towards mite and pollen allergen extracts in vitro
| Autor: | Søren Hansen, Peter Adler Würtzen, Valery R Wagtmann, Dick Papazian |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Allergy
Th1 Cells/drug effects Cell Communication/drug effects Cell Communication Dendritic cells Cell Proliferation/drug effects Immunology and Allergy Cytotoxic T cell Th1-Th2 Balance Plant Proteins Interleukin-13 Interleukin-13/biosynthesis Translational Interleukin respiratory system medicine.anatomical_structure Pollen Human Hypersensitivity/immunology Th2 Cells/drug effects Plant Proteins/immunology T cell Immunology education T cells Antigens Plant/immunology Pollen/immunology Respiratory Mucosa Biology Culture Media Conditioned/pharmacology Cell Line Mucosa Dendritic Cells/drug effects Epithelial Cells/drug effects Allergens/immunology Immune system Th2 Cells Th1-Th2 Balance/drug effects Antigen Respiratory Mucosa/drug effects medicine Hypersensitivity Immune Tolerance Animals Humans Antigens Dermatophagoides Immunity Mucosal Cell Proliferation House dust mite Antigens Dermatophagoides/immunology Plant Extracts Interleukin-5/biosynthesis Plant Extracts/pharmacology Epithelial Cells Dendritic Cells Allergens Antigens Plant Th1 Cells biology.organism_classification medicine.disease Coculture Techniques Culture Media Conditioned Respiratory epithelium Interleukin-5 Tolerance |
| Zdroj: | Papazian, D, Wagtmann, V R, Hansen, S & Würtzen, P A 2015, ' Direct contact between dendritic cells and bronchial epithelial cells inhibits T cell recall responses towards mite and pollen allergen extracts in vitro ', Clinical and Experimental Immunology, vol. 181, no. 2, pp. 207-218 . https://doi.org/10.1111/cei.12611 |
| DOI: | 10.1111/cei.12611 |
| Popis: | Summary Airway epithelial cells (AECs) form a polarized barrier along the respiratory tract. They are the first point of contact with airborne antigens and are able to instruct resident immune cells to mount appropriate immune responses by either soluble or contact-dependent mechanisms. We hypothesize that a healthy, polarized epithelial cell layer inhibits inflammatory responses towards allergens to uphold homeostasis. Using an in-vitro co-culture model of the airway epithelium, where a polarized cell layer of bronchial epithelial cells can interact with dendritic cells (DCs), we have investigated recall T cell responses in allergic patients sensitized to house dust mite, grass and birch pollen. Using allergen extract-loaded DCs to stimulate autologous allergen-specific T cell lines, we show that AEC-imprinted DCs inhibit T cell proliferation significantly of Bet v 1-specific T cell lines as well as decrease interleukin (IL)-5 and IL-13 production, whereas inhibition of Phl p 5-specific T cells varied between different donors. Stimulating autologous CD4+ T cells from allergic patients with AEC-imprinted DCs also inhibited proliferation significantly and decreased production of both T helper type 1 (Th1) and Th2 cytokines upon rechallenge. The inhibitory effects of AECs’ contact with DCs were absent when allergen extract-loaded DCs had been exposed only to AECs supernatants, but present after direct contact with AECs. We conclude that direct contact between DCs and AECs inhibits T cell recall responses towards birch, grass and house dust mite allergens in vitro, suggesting that AECs-DC contact in vivo constitute a key element in mucosal homeostasis in relation to allergic sensitisation. |
| Databáze: | OpenAIRE |
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