TARGET OF RAPAMYCIN signaling plays a role in Arabidopsis growth promotion by Azospirillum brasilense Sp245
| Autor: | César Arturo Peña-Uribe, Manuel Méndez-Gómez, José López-Bucio, Homero Reyes de la Cruz, Ernesto García-Pineda, Elda Castro-Mercado |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Meristem Arabidopsis Plant Development Plant Science Azospirillum brasilense Root hair 01 natural sciences Plant Roots 03 medical and health sciences Phosphatidylinositol 3-Kinases Auxin Rhizobiaceae Genetics Arabidopsis thaliana Phosphorylation chemistry.chemical_classification biology Indoleacetic Acids Arabidopsis Proteins fungi Lateral root food and beverages General Medicine Triazoles biology.organism_classification TOR signaling Cell biology 030104 developmental biology chemistry Quinolines Agronomy and Crop Science 010606 plant biology & botany Signal Transduction |
| Zdroj: | Plant science : an international journal of experimental plant biology. 293 |
| ISSN: | 1873-2259 |
| Popis: | Azospirillum brasilense colonizes plant roots and improves productivity, but the molecular mechanisms behind its phytostimulation properties remain mostly unknown. Here, we uncover an important role of TARGET OF RAPAMYCIN (TOR) signaling on the response of Arabidopsis thaliana to A. brasilense Sp245. The effect of the bacterium on TOR expression was analyzed in the transgenic line TOR/tor-1, which carries a translational fusion with the GUS reporter protein, and the activity of TOR was assayed thought the phosphorylation of its downstream signaling target S6K protein. Besides, the role of TOR on plant growth in inoculated plants was assessed using the ATP-competitive inhibitor AZD-8055. A decrease in growth of the primary root correlates with an improved branching and absorptive capacity via lateral root and root hair proliferation 6 days after transplant to different concentrations of the bacterium (103 or 105 CFU/mL). Bacterization increased the expression of TOR in shoot and root apexes and promoted phosphorylation of S6K 3 days after transplant. The TOR inhibitor AZD-8055 (1 μM) inhibited plant growth and cell division in root meristems and in lateral root primordia, interfering with the phytostimulation by A. brasilense. In addition, the role of auxin produced by the bacterium to stimulate TOR expression was explored. Noteworthy, the A. brasilense mutant FAJ009, impaired in auxin production, was unable to elicit TOR signaling to the level observed for the wild-type strain, showing the importance of this phyhormone to stimulate TOR signaling. Together, our findings establish an important role of TOR signaling for the probiotic traits elicited by A. brasilense in A. thaliana. |
| Databáze: | OpenAIRE |
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