High-throughput fabrication of vascularized adipose microtissues for 3D bioprinting
| Autor: | Chris Vercruysse, Florian Vanlauwe, Liesbeth Tytgat, Lise De Moor, Elisabeth De Maere, Phillip Blondeel, Michelle Ryx, Sandra Van Vlierberghe, Peter Dubruel, Lara Benmeridja, Heidi Declercq |
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| Přispěvatelé: | Applied Physics and Photonics |
| Rok vydání: | 2020 |
| Předmět: |
Male
0206 medical engineering Biomedical Engineering Medicine (miscellaneous) Adipose tissue 02 engineering and technology law.invention Biomaterials 03 medical and health sciences STEM-CELLS TISSUE SPHEROIDS law In vivo Lipid droplet Human Umbilical Vein Endothelial Cells Humans 030304 developmental biology 0303 health sciences 3D bioprinting Tissue Engineering Chemistry Stem Cells Spheroid Bioprinting Middle Aged 020601 biomedical engineering Coculture Techniques Transplantation Adipose Tissue Adipogenesis embryonic structures Microvessels Printing Three-Dimensional Female Stem cell Biomedical engineering |
| Zdroj: | Journal of tissue engineering and regenerative medicineREFERENCES. 14(6) |
| ISSN: | 1932-7005 |
| Popis: | For patients with soft tissue defects, repair with autologous in vitro engineered adipose tissue could be a promising alternative to current surgical therapies. A volume-persistent engineered adipose tissue construct under in vivo conditions can only be achieved by early vascularization after transplantation. The combination of 3D bioprinting technology with self-assembling microvascularized units as building blocks can potentially answer the need for a microvascular network. In the present study, co-culture spheroids combining adipose-derived stem cells (ASC) and human umbilical vein endothelial cells (HUVEC) were created with an ideal geometry for bioprinting. When applying the favourable seeding technique and condition, compact viable spheroids were obtained, demonstrating high adipogenic differentiation and capillary-like network formation after 7 and 14 days of culture, as shown by live/dead analysis, immunohistochemistry and RT-qPCR. Moreover, we were able to successfully 3D bioprint the encapsulated spheroids, resulting in compact viable spheroids presenting capillary-like structures, lipid droplets and spheroid outgrowth after 14 days of culture. This is the first study that generates viable high-throughput (pre-)vascularized adipose microtissues as building blocks for bioprinting applications using a novel ASC/HUVEC co-culture spheroid model, which enables both adipogenic differentiation while simultaneously supporting the formation of prevascular-like structures within engineered tissues in vitro. |
| Databáze: | OpenAIRE |
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