Effects of the Hippo Signaling Pathway in Human Gastric Cancer

Autor: Zibin Tian, Guang-Xi Zhou, Qi Zhang, Kun Yang, Chang-Hu Xue, Kun Zhao, Cui-Ping Zhang, Xiaoyu Li
Rok vydání: 2013
Předmět:
Gastritis
Atrophic
Cancer Research
MST1
Epidemiology
Blotting
Western
Apoptosis
Protein Serine-Threonine Kinases
Biology
Metastasis
Stomach Neoplasms
Proto-Oncogene Proteins
medicine
Humans
CDX2 Transcription Factor
Hippo Signaling Pathway
RNA
Messenger
Adaptor Proteins
Signal Transducing
Cell Proliferation
Homeodomain Proteins
YAP1
Regulation of gene expression
Metaplasia
Hippo signaling pathway
Hepatocyte Growth Factor
Carcinoma
Public Health
Environmental and Occupational Health
Nuclear Proteins
TEA Domain Transcription Factors
Cancer
YAP-Signaling Proteins
Middle Aged
Phosphoproteins
medicine.disease
Immunohistochemistry
digestive system diseases
Up-Regulation
DNA-Binding Proteins
Gene Expression Regulation
Neoplastic
Real-time polymerase chain reaction
Oncology
Hippo signaling
Case-Control Studies
Cancer research
Octamer Transcription Factor-3
Precancerous Conditions
Acyltransferases
Signal Transduction
Transcription Factors
Zdroj: Asian Pacific Journal of Cancer Prevention. 14:5199-5205
ISSN: 1513-7368
Popis: Background/Aim: The Hippo signaling pathway is a newly discovered and conserved signaling cascade, which regulates organ size control by governing cell proliferation and apoptosis. This study aimed to investigate its effects in human gastric cancer. Methods: Tumor tissues (n=60), adjacent non-tumor tissues (n=60) and normal tissues (n=60) were obtained from the same patients with primary gastric cancer (GC). In addition, 70 samples of chronic atrophic gastritis (CAG) tissues were obtained from patients with intestinal metaplasia (IM) by endoscopic biopsy. Hippo signaling molecules, including Mst1, Lats1, YAP1, TAZ, TEAD1, Oct4 and CDX2, were determined by quantitative polymerase chain reaction (qPCR). Protein expression of Mst1, Lats1, YAP1, TEAD1 and CDX2 was assessed by immunohistochemistry and Western blotting. Results: Mst1, Lats1 and Oct4 mRNA expression showed an increasing tendency from GC tissues to normal gastric tissues, while the mRNA expression of YAP1, TAZ and TEAD1 was up-regulated (all P
Databáze: OpenAIRE
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