Development and Validation of a Column High-Performance Liquid Chromatography Method for Quantification of Ganaphaliin A and B in Inflorescences of Gnaphalium liebmannii Sch. Bp ex Klatt
| Autor: | Gabriela Rubí Tapia-Álvarez, Fernando Rodríguez-Ramos, Andrés Navarrete, Víctor H Sánchez-Estrada, Alejandro Alfaro-Romero |
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| Rok vydání: | 2011 |
| Předmět: |
Flavonoids
Pharmacology chemistry.chemical_classification Gnaphaliin Gnaphalium Chromatography Molecular Structure biology Chemistry Analytical chemistry Reproducibility of Results Flowers Repeatability biology.organism_classification High-performance liquid chromatography Flavones Analytical Chemistry Photodiode array detector Environmental Chemistry Hplc method Agronomy and Crop Science Quantitative analysis (chemistry) Chromatography High Pressure Liquid Food Science |
| Zdroj: | ResearcherID Scopus-Elsevier |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoac/94.4.1076 |
| Popis: | An HPLC method was developed for the simultaneous determination of gnaphaliin A and B, active compounds of Gnaphalium liebmannii Sch. Bp ex Klatt. The HPLC separation was performed on an Inertsil ODS-3 (150 × 4.6 mm id, 5 μm) RP C18 column operated at 40°C; the isocratic mobile phase was 0.02% aqueous orthophosphoric acid– methanol–acetonitrile (50 + 30 + 20, v/v/v), with a run time of 20 min and flow rate of 1.5 mL/min. Detection with a photodiode array detector (PDAD) was at 270 nm. The method was validated for linearity, repeatability, LOD, and LOQ. The LOD and LOQ for gnaphaliin A and B were found to be in the range of 0.4–0.5 and 1.0–1.4 μg/mL, respectively. This is the frst report of an analytical method developed for the quantitative analysis of flavones from Gnaphalium species by HPLC-PDAD with applications for raw material and commercial products. |
| Databáze: | OpenAIRE |
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