Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
| Autor: | Ronald L. Gross, Scott F. Basinger, Jun-Hai Yang, Samuel M. Wu |
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| Rok vydání: | 2003 |
| Předmět: |
Light
Antimycin A Urodela 3 3'-Diaminobenzidine Mitochondrion Biology medicine.disease_cause Membrane Potentials Electron Transport chemistry.chemical_compound Organ Culture Techniques Rotenone medicine Animals chemistry.chemical_classification Membrane potential Reactive oxygen species Rhodamines Anatomy Fluoresceins Electron transport chain Fluorescence Mitochondria Light intensity chemistry Biophysics sense organs Reactive Oxygen Species Oxidative stress Photoreceptor Cells Vertebrate |
| Zdroj: | Investigative ophthalmologyvisual science. 44(3) |
| ISSN: | 0146-0404 |
| Popis: | Purpose To investigate whether photoreceptor ellipsoids generate reactive oxygen species (rOx) after blue light illumination. Methods Cultured salamander photoreceptors were exposed to blue light (480 +/- 10 nm; 10 mW/cm(2)). The light-induced catalytic redox activity in the culture was monitored with the use of 3,3'-diaminobenzidine (DAB). Tetramethylrhodamine ethyl ester (TMRE) and 2',7'-dichlorodihydro-fluorescein acetate (DHF-DA) were used as probes to measure the mitochondrial membrane potential and intracellular rOx, respectively. Results A significant deposit of DAB polymers was found in the culture after exposure to blue light. Basal levels of rOx were observed in photoreceptor ellipsoids when cells were stained with DHF-DA. This staining colocalized with TMRE. After exposure to blue light, a sharp increase of rOx immediately occurred in the ellipsoids of most photoreceptors. When the light intensity was reduced, the response kinetics of rOx generation were slowed down; however, comparable amounts of rOx were generated after a standard time of exposure to light. The production of rOx in photoreceptors was markedly decreased when an antioxidant mixture was included in the medium during exposure to light. Rotenone or antimycin A, the respiratory electron transport blockers at complex I and III, respectively, significantly suppressed the light-evoked generation of rOx. Conclusions A robust amount of rOx is produced in the ellipsoid when photoreceptors are exposed to blue light. This light-induced effect is antioxidant sensitive and strongly coupled to mitochondrial electron transport. The cumulative effect of light on rOx generation over time may implicate a role for mitochondria in light-induced oxidative damage of photoreceptors. |
| Databáze: | OpenAIRE |
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