L-carnitine mitigates UVA-induced skin tissue injury in rats through downregulation of oxidative stress, p38/c-Fos signaling, and the proinflammatory cytokines
| Autor: | Ibrahim A. Maghrabi, Majed M. Al Robaian, Samir A. Salama, Hany A. Omar, Hany H. Arab, Gamil M. Abd-Allah, Hesham S. Gad |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine MAP Kinase Signaling System Ultraviolet Rays DNA damage Down-Regulation 8-Oxo-2'-deoxyguanosine Apoptosis Pharmacology Toxicology medicine.disease_cause Protein oxidation Proinflammatory cytokine Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Carnitine medicine Animals Skin biology General Medicine Glutathione Rats Proliferating cell nuclear antigen Oxidative Stress 030104 developmental biology chemistry 030220 oncology & carcinogenesis biology.protein Cytokines Lipid Peroxidation sense organs Proto-Oncogene Proteins c-fos Immunosuppressive Agents Oxidative stress DNA Damage Signal Transduction |
| Zdroj: | Chemico-Biological Interactions. 285:40-47 |
| ISSN: | 0009-2797 |
| DOI: | 10.1016/j.cbi.2018.02.034 |
| Popis: | UVA comprises more than 90% of the solar UV radiation reaching the Earth. Artificial lightening lamps have also been reported to emit significant amounts of UVA. Exposure to UVA has been associated with dermatological disorders including skin cancer. At the molecular level, UVA damages different cellular biomolecules and triggers inflammatory responses. The current study was devoted to investigate the potential protective effect of L-carnitine against UVA-induced skin tissue injury using rats as a mammalian model. Rats were distributed into normal control group (NC), L-carnitine control group (LC), UVA-Exposed group (UVA), and UVA-Exposed and L-carnitine-treated group (UVA-LC). L-carnitine significantly attenuated UVA-induced elevation of the DNA damage markers 8-oxo-2'-deoxyguanosine (8-oxo-dG) and cyclobutane pyrimidine dimers (CPDs) as well as decreased DNA fragmentation and the activity of the apoptotic marker caspase-3. In addition, L-carnitine substantially reduced the levels of lipid peroxidation marker (TBARS) and protein oxidation marker (PCC) and significantly elevated the levels of the total antioxidant capacity (TAC) and the antioxidant reduced glutathione (GSH) in the skin tissues. Interestingly, L-carnitine upregulated the level of the DNA repair protein proliferating cell nuclear antigen (PCNA). Besides it mitigated the UVA-induced activation of the oxidative stress-sensitive signaling protein p38 and its downstream target c-Fos. Moreover, L-carnitine significantly downregulated the levels of the early response proinflammatory cytokines TNF-α, IL-6, and IL-1β. Collectively, our results highlight, for the first time, the potential attenuating effects of L-carnitine on UVA-induced skin tissue injury in rats that is potentially mediated through suppression of UVA-induced oxidative stress and inflammatory responses. |
| Databáze: | OpenAIRE |
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