Insulin Induces Dephosphorylation of Eukaryotic Initiation Factor 2α and Restores Protein Synthesis in Vulnerable Hippocampal Neurons after Transient Brain Ischemia
| Autor: | José A. Rafols, Gary S. Krause, Katie R. Hikade, Nabil J. Bahu, Sarah S. Alousi, Jonathon M. Sullivan, Blaine C. White |
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| Rok vydání: | 1999 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Eukaryotic Initiation Factor-2 Nerve Tissue Proteins Hippocampal formation Hippocampus 030218 nuclear medicine & medical imaging Dephosphorylation Brain ischemia 03 medical and health sciences 0302 clinical medicine Eukaryotic initiation factor Internal medicine medicine Animals Hypoglycemic Agents Insulin Rats Long-Evans Phosphorylation Neurons eIF2 business.industry Dentate gyrus medicine.disease Rats Endocrinology Neurology Ischemic Attack Transient Autoradiography Neurology (clinical) Cardiology and Cardiovascular Medicine business 030217 neurology & neurosurgery Immunostaining |
| Zdroj: | Journal of Cerebral Blood Flow & Metabolism. 19:1010-1019 |
| ISSN: | 1559-7016 0271-678X |
| DOI: | 10.1097/00004647-199909000-00009 |
| Popis: | Brain reperfusion causes prompt, severe, and prolonged protein synthesis suppression and increased phosphorylation of eukaryotic initiation factor 2α [eIF2α(P)] in hippocampal CA1 and hilar neurons, The authors hypothesized that eIF2α(P) dephosphorylation would lead to recovery of protein synthesis. Here the effects of insulin, which activates phosphatases, were examined by immunostaining for eIF2α(P) and autoradiography of in vivo35S amino acid incorporation. Rats resuscitated from a 10-minute cardiac arrest were given 0, 2, 10 or 20U/kg of intravenous insulin, underwent reperfusion for 90 minutes, and were perfusion fixed. Thirty minutes before perfusion fixation, control and resuscitated animals received 500 μCi/kg of 35S methionine/cysteine. Alternate 30-μm brain sections were autoradiographed or immunostained for eIF2α(P). Controls had abundant protein synthesis and no eIF2α(P) in hippocampal neurons. Untreated reperfused neurons in the CA1, hilus, and dentate gyrus had intense staining for eIF2α(P) and reduced protein synthesis; there was little improvement with treatment with 2 or 10 U/kg of insulin. However, with 20 U/kg of insulin, these neurons recovered protein synthesis and were free of eIF2α(P). These results show that the suppression of protein synthesis in the reperfused brain is reversible; they support a causal association between eIF2α(P) and inhibition of protein synthesis, and suggest a mechanism for the neuroprotective effects of insulin. |
| Databáze: | OpenAIRE |
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